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Calcium carbonate microparticles containing a photosensitizer photosens: Preparation, ultrasound stimulated dye release, and in vivo application

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Abstract

Calcium carbonate microparticles with a size of 0.9 ± 0.2 μm containing a photosensitizer Photosens in a concentration of 2.0 ± 0.2 wt % were prepared by ultrasound-stimulated coprecipitation (20 kHz, 1W/cm2). It is shown that the encapsulated photosensitizer can be released by ultrasonic irradiation (0.89MHz, 1 W/cm2, 5 min) as a result of the destruction and recrystallization of calcium carbonate micro-particles. It is established that the combined ultrasonic (0.89 MHz, 1 W/cm2) and light (670 nm, 10 mW/cm2) in vivo influence on the transferred PC-1 strain tumors of rat liver containing intratumorally injected micro-containers with a photosensitizer gives rise to dystrophic changes in tumor cells and to the appearance of extensive necrotic centers, pointing to the presence of the evident destructive effect. Such microcontainers are proposed for use in treating external tumors or tumors accessible for ultrasonic and optical irradiation.

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Authors and Affiliations

  1. Chernyshevsky State University, ul. Astrakhanskaya 83, Saratov, 410012, Russia

    Yu. I. Svenskaya, G. S. Terentyuk, A. O. Kuz’mina, M. M. Burashnikova & D. A. Gorin

  2. Razumovsky State Medical University, ul. B. Kazach’ya 112, Saratov, 410012, Russia

    N. A. Navolokin, A. B. Bucharskaya, G. S. Terentyuk & G. N. Maslyakova

  3. Ulyanovsk State University, ul. L. Tolstogo 42, Ulyanovsk, 432017, Russia

    G. S. Terentyuk

  4. Organic Intermediates and Dyes Institute, ul. B. Sadovaya 1, korp. 4, Moscow, 123995, Russia

    E. A. Lukyanets

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  1. Yu. I. Svenskaya
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Correspondence to Yu. I. Svenskaya.

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Original Russian Text © Yu.I. Svenskaya, N.A. Navolokin, A.B. Bucharskaya, G.S. Terentyuk, A.O. Kuz’mina, M.M. Burashnikova, G.N. Maslyakova, E.A. Lukyanets, D.A. Gorin, 2014, published in Rossiiskie Nanotekhnologii, 2014, Vol. 9, Nos. 7–8.

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Svenskaya, Y.I., Navolokin, N.A., Bucharskaya, A.B. et al. Calcium carbonate microparticles containing a photosensitizer photosens: Preparation, ultrasound stimulated dye release, and in vivo application. Nanotechnol Russia 9, 398–409 (2014). https://doi.org/10.1134/S1995078014040181

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