The effect of silicon dioxide nanoparticles on the expression of key genes of the brain serotonergic (5-HT) system has been studied in specific pathogen-free mice chronically exposed to Tarkosil nanoaerosol (25-nm particles). Males of the Balb/c and C57Bl/6 strains are used to reveal genetic differences in the response to the nanomaterial. The animals are exposed to aerosol with a mean particle size of 107 nm for 10 days. After exposure, their midbrains, hippocampi, and frontal cortices are examined. The expression rates of genes for 5-HT1A and 5-HT2A receptors; tryptophan hydroxylase (TPH2), the key enzyme in 5-HT production; serotonin transporter (5-HTT); and interleukin 6 (IL6) as a marker of inflammation in the brain are assayed by quantitative reverse transcription PCR. The expression of 5-HT1A is notably elevated in the hippocampus of Balb/c mice, but it tends to decrease in C57Bl/6. No changes in the expression of the genes for 5-HT2A, TPH2, 5-HTT, or IL6 are noted in any brain divisions. Thus, the chronic inhalation of silicon dioxide nanoparticles does not activate nonspecific immunity in the brain, but it exerts contrasting genotype-specific effects on the expression of the 5-HT1A receptor in the hippocampus.
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G. Oberdörster, Z. Sharp, V. Atudorei, A. Elder, R. Gelein, W. Kreyling, and C. Cox, “Translocation of inhaled ultrafine particles to the brain,” Inhalation. Toxicol. 16(6–7), 437–445 (2004).
A. Elder, R. Gelein, V. Silva, T. Feikert, L. Opanashuk, J. Carter, R. Potter, A. Maynard, Y. Ito, J. Finkelstein, and G. Oberdorster, “Translocation of inhaled ultrafine manganese oxide particles to the central nervous system,” Environ. Health. Perspect. 114, 1172–1178 (2006).
R. G. Lucchini, D. C. Dorman, A. Elder, and B. Veronesi, “Neurological impacts from inhalation of pollutants and the nose-brain connection,” Neurotoxicology 33(4), 838–841 (2012).
H. S. Sharma and A. Sharma, “Nanowired drug delivery for neuroprotection in central nervous system injuries: modulation by environmental temperature, intoxication of nanoparticles, and comorbidity factors,” WIREs Nanomed. Nanobiotechnol. 4, 184–203 (2012).
Y. Song and S. Tang, “Nanoexposure, unusual diseases, and new health and safety concerns,” Sci. World J. 11, 1821–1828 (2011).
H. S. Sharma and A. Sharma, “Breakdown of the blood-brain barrier in stress alters cognitive dysfunction and induces brain pathology. New perspective for neuroprotective strategies,” in Brain Protection in Schizophrenia, Mood and Cognitive Disorders, Ed. by M. Ritsner (Springer-Verlag, Berlin, New-York, 2010), pp. 243–304.
H. S. Sharma, “Nanoneuroscience: emerging concepts on nanoneurotoxicity and nanoneuroprotection,” Nanomedicine (London) 2(6), 753–758 (2007).
H. S. Sharma, R. Patnaik, A. Sharma, P. O. Sjoquist, and J. V. Lafuente, “Silicon dioxide nanoparticles (SiO2, 40–50 nm) exacerbate pathophysiology of traumatic spinal cord injury and deteriorate functional outcome in the rat. An experimental study using pharmacological and morphological approaches,” J. Nanosci. Nanotechnol. 9(8), 4970–4980 (2009).
J. V. Lafuente, A. Sharma, R. Patnaik, D. F. Muresanu, and H. S. Sharma, “Diabetes exacerbates nanoparticles induced brain pathology,” CNS Neurol. Disord. Drug. Targets 11(1), 26–39 (2012).
M. Chen and A. von Mikecz, “Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO2 nanoparticles,” Exp. Cell Res. 305(1), 51–62 (2005).
M. A. Malvindi, V. Brunetti, G. Vecchio, A. Galeone, R. Cingolani, and P. P. Pompa, “SiO2 nanoparticles biocompatibility and their potential for gene delivery and silencing,” Nanoscale 4(2), 486–495 (2012).
J. Pekkanen, K. L. Timonen, J. Ruuskanen, A. Reponen, and A. Mirme, “Effects of ultrafine and fine particles in urban air on peak expiratory flow among children with asthmatic symptoms,” Environ. Res. 74, 24–33 (1997).
G. Oberdörster, V. Stone, and K. Donaldson, “Toxicology of nanoparticles: a historical perspective,” Nanotoxicology 1(1), 2–25 (2007).
B. L. Jacobs and C. A. Fornal, “Serotonin and behavior. A general hypothesis,” in Psychopharmacology: The Fourth Generation of Progress, Ed. by F. E. Bloom and D. J. Kupfer (Raven Press, New York, 1995), pp. 461–469.
I. Lucki, “The spectrum of behaviors influenced by serotonin,” Biol. Psychiatry 44(3), 151–162 (1998).
T. G. Amstislavskaya and N. K. Popova, “The roles of different types of serotonin receptors in activation of the hypophyseal-testicular complex induced in mice by the presence of a female,” Neurosci. Behav. Physiol. 34(8), 833–837 (2004).
M. A. Tikhonova, T. G. Amstislavskaya, and A. V. Kulikov, “Chronic administration of imipramine normalizes decreased sexual motivation and increased predisposition to catalepsy induced by propylthiouracil in rats,” Neurosci. Behav. Physiol. 39(4), 409–415 (2009).
N. K. Popova and V. S. Naumenko, “5-HT1A receptor as a key player in the brain 5-HT system,” Rev. Neurosci. 24(2), 191–204 (2013).
B. L. Jacobs and E. C. Azmitia, “Structure and function of the brain serotonin system,” Physiol. Rev. 72, 165–229 (1992).
A. Meneses and G. Liy-Salmeron, “Serotonin and emotion, learning and memory,” Rev. Neurosci. 23(5–6), 543–553 (2012).
P. Chomczynski and N. Sacchi, “Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,” Anal. Biochem. 162, 156–159 (1987).
P. Blier and C. de Montigny, “Current advances and trends in the treatment of depression,” Trends Pharmacol. Sci. 15(7), 220–226 (1994).
S. K. Sohaebuddin, P. T. Thevenot, D. Baker, J. W. Eaton, and L. Tang, “Nanomaterial cytotoxicity is composition, size, and cell type dependent,” Part. Fibre Toxicol. 7, 22 (2010).
D. Napierska, L. C. Thomassen, D. Lison, J. A. Martens, and P. H. Hoet, “The nanosilica hazard: another variable entity,” Part. Fibre. Toxicol. 7(1), 39 (2010).
D. Napierska, L. C. Thomassen, V. Rabolli, D. Lison, L. Gonzalez, M. Kirsch-Volders, J. A. Martens, and P. H. Hoet, “Size-dependent cytotoxicity of monodisperse silica nanoparticles in human endothelial cells,” Small 5(7), 846–853 (2009).
S. P. Hudson, R. F. Padera, R. Langer, and D. S. Kohane, “The biocompatibility of mesoporous silicates,” Biomaterials 29(30), 4045–4055 (2008).
Y. Dwivedi, A. C. Mondal, G. V. Payappagoudar, and H. S. Rizavi, “Differential regulation of serotonin (5HT)2A receptor mRNA and protein levels after single and repeated stress in rat brain: role in learned helplessness behavior,” Neuropharmacology 48(2), 204–214 (2005).
S. Maswood, J. E. Barter, L. R. Watkins, and S. F. Maier, “Exposure to inescapable but not escapable shock increases extracellular levels of 5HT in the dorsal raphe nucleus of the rat,” Brain. Res. 783, 115–120 (1998).
S. Wissink, O. Meijer, D. Pearce, B. van Der Burg, and P. T. van Der Saag, “Regulation of the rat serotonin-1A receptor gene by corticosteroids,” J. Biol. Chem. 275(2), 1321–1326 (2000).
S. E. Pel’tek, M. P. Moshkin, L. A. Gerlinskaya, T. N. Goryachkovskaya, S. V. Bannikova, O. A. Podkolodnaya, V. M. Popik, and N. A. Kolchanov, “SPF-vivarium is a scientific-technical complex for researches in the area of nanosafety,” Nanotekhnol. Ekol. Proizv. 2(15) (2012).
M. P. Moshkin, S. E. Pel’tek, L. A. Gerlinskaya, T. N. Goryachkovskaya, G. V. Kontsevaya, S. O. Maslennikova, V. V. Popik, N. A. Kolchanov, et al., “Acute immune response to the intranasal application of nanoparticles of SiO2 (tarkosil 25) in mice of two strains,” Nanotech. Russ. 6(11–12), 763 (2011).
A. V. Kulikov, V. S. Naumenko, A. S. Tsybko, N. A. Sinyakova, D. V. Bazovkina, and N. K. Popova, “Role of glycoprotein gp130 in serotonin mediator system of mice brain,” Izv. Akad. Nauk SSSR Ser. Biol. 44(5), 904–910 (2010).
Original Russian Text © A.S. Tsybko, T.G. Amstislavskaya, G.V. Kontsevaya, L.A. Gerlinskaya, 2014, published in Rossiiskie Nanotekhnologii, 2014, Vol. 9, Nos. 3–4.
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Tsybko, A.S., Amstislavskaya, T.G., Kontsevaya, G.V. et al. Effect of chronic inhalation of silicon dioxide nanoparticles (Tarkosil 25) on the expression of key genes of the serotonergic system in the mouse brain. Nanotechnol Russia 9, 213–218 (2014). https://doi.org/10.1134/S1995078014020177
- Frontal Cortex
- Tryptophan Hydroxylase
- Silicon Nanoparticles
- Guanidine Thiocyanate
- Blood Barrier