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Effect of chronic inhalation of silicon dioxide nanoparticles (Tarkosil 25) on the expression of key genes of the serotonergic system in the mouse brain

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Abstract

The effect of silicon dioxide nanoparticles on the expression of key genes of the brain serotonergic (5-HT) system has been studied in specific pathogen-free mice chronically exposed to Tarkosil nanoaerosol (25-nm particles). Males of the Balb/c and C57Bl/6 strains are used to reveal genetic differences in the response to the nanomaterial. The animals are exposed to aerosol with a mean particle size of 107 nm for 10 days. After exposure, their midbrains, hippocampi, and frontal cortices are examined. The expression rates of genes for 5-HT1A and 5-HT2A receptors; tryptophan hydroxylase (TPH2), the key enzyme in 5-HT production; serotonin transporter (5-HTT); and interleukin 6 (IL6) as a marker of inflammation in the brain are assayed by quantitative reverse transcription PCR. The expression of 5-HT1A is notably elevated in the hippocampus of Balb/c mice, but it tends to decrease in C57Bl/6. No changes in the expression of the genes for 5-HT2A, TPH2, 5-HTT, or IL6 are noted in any brain divisions. Thus, the chronic inhalation of silicon dioxide nanoparticles does not activate nonspecific immunity in the brain, but it exerts contrasting genotype-specific effects on the expression of the 5-HT1A receptor in the hippocampus.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Ak. Lavrent’eva 10, Novosibirsk, 630090, Russia

    A. S. Tsybko, T. G. Amstislavskaya, G. V. Kontsevaya & L. A. Gerlinskaya

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  1. A. S. Tsybko
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  2. T. G. Amstislavskaya
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  3. G. V. Kontsevaya
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  4. L. A. Gerlinskaya
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Correspondence to A. S. Tsybko.

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Original Russian Text © A.S. Tsybko, T.G. Amstislavskaya, G.V. Kontsevaya, L.A. Gerlinskaya, 2014, published in Rossiiskie Nanotekhnologii, 2014, Vol. 9, Nos. 3–4.

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Tsybko, A.S., Amstislavskaya, T.G., Kontsevaya, G.V. et al. Effect of chronic inhalation of silicon dioxide nanoparticles (Tarkosil 25) on the expression of key genes of the serotonergic system in the mouse brain. Nanotechnol Russia 9, 213–218 (2014). https://doi.org/10.1134/S1995078014020177

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  • DOI: https://doi.org/10.1134/S1995078014020177

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