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Fluorescently labeled single-walled carbon nanotubes and their hybrids with oligonucleotides

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Abstract

The approach to the preparation of fluorescein-labeled single-walled carbon nanotubes was proposed. The investigation of physico-chemical properties of obtained modified nanotubes was performed. The method of the design of non-covalent hybrids of fluorescein-labeled carbon nanotubes with nucleic acid fragments containing pyrene residues at 5′-termini was developed. This method is based on stacking interactions of pyrene residues with carbon nanotubes surface. The effect of carbon nanotubes functionalization type on the efficacy of sorption of pyrene conjugates of oligonucleotides was examined.

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Abbreviations

CNT:

carbon nanotube

DMF:

N,N-dimethylformamide

EDC:

1-ethyl-3-[3-(dimethylamino)-propyl]carbodiimide

FITC:

fluorescein isothiocyanate

HMDA:

hexamethylenediamine

PAMAM:

polyamidoamine dendrimer

SWCNT:

single-walled carbon nanotube

TEA:

triethylamine

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Akad. Lavrentieva, 8, Novosibirsk, 8630090, Russia

    E. K. Apartsin, D. S. Novopashina & A. G. Ven’yaminova

  2. Novosibirsk State University, ul. Pirogova, 2, Novosibirsk, 630090, Russia

    E. K. Apartsin

  3. Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, pr. Akad. Lavrentieva, 13, Novosibirsk, 630090, Russia

    Yu. V. Nastaushev

Authors
  1. E. K. Apartsin
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  2. D. S. Novopashina
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  3. Yu. V. Nastaushev
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  4. A. G. Ven’yaminova
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Corresponding author

Correspondence to D. S. Novopashina.

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Original Russian Text © E.K. Apartsin, D.S. Novopashina, Yu.V. Nastaushev, A.G. Ven’yaminova, 2012, published in Rossiiskie Nanotekhnologii, 2012, Vol. 7, Nos. 3–4.

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Apartsin, E.K., Novopashina, D.S., Nastaushev, Y.V. et al. Fluorescently labeled single-walled carbon nanotubes and their hybrids with oligonucleotides. Nanotechnol Russia 7, 99–109 (2012). https://doi.org/10.1134/S1995078012020024

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  • DOI: https://doi.org/10.1134/S1995078012020024

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