Nanotechnologies in Russia

, Volume 4, Issue 11–12, pp 876–880 | Cite as

Application of atomic-force microscopy technology to a structural analysis of the mitochondrial inner membrane

  • E. V. DubrovinEmail author
  • T. N. Murugova
  • K. A. Motovilov
  • L. S. Yaguzhinskii
  • I. V. Yaminsky


For the first time, the surface of mitoplasts (mitochondria devoid of an outer membrane) was investigated by atomic-force microscopy (AFM). AFM has revealed folds on the surface of mitoplasts with thicknesses of 30–40 nm, which coincides with that of “dry cristae” in mitochondria measured with small-angle neutron scattering and electron microscopy. These results indicate the existence of a specific system maintaining the configuration of a mitoplast membrane similar to that of the inner membrane of intact mitochondria under their swelling in hypotonic conditions. The prospects of using AFM to study the configuration of the surface of the mitochondrial inner membrane and other biological membrane systems were demonstrated.


Osmotic Shock Small Angle Neutron Scattering Hypotonic Medium Electron Microscopy Photo Hypotonic Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • E. V. Dubrovin
    • 1
    Email author
  • T. N. Murugova
    • 2
  • K. A. Motovilov
    • 3
    • 4
  • L. S. Yaguzhinskii
    • 4
  • I. V. Yaminsky
    • 1
  1. 1.Faculty of PhysicsMoscow State UniversityMoscowRussia
  2. 2.Frank Laboratory of Neutron PhysicsJoint Institute for Nuclear ResearchDubnaRussia
  3. 3.Faculty of ChemistryMoscow State UniversityMoscowRussia
  4. 4.Belozersky Scientific-Research Institute of Physical and Chemical BiologyMoscow State UniversityMoscowRussia

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