Application of atomic-force microscopy technology to a structural analysis of the mitochondrial inner membrane
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For the first time, the surface of mitoplasts (mitochondria devoid of an outer membrane) was investigated by atomic-force microscopy (AFM). AFM has revealed folds on the surface of mitoplasts with thicknesses of 30–40 nm, which coincides with that of “dry cristae” in mitochondria measured with small-angle neutron scattering and electron microscopy. These results indicate the existence of a specific system maintaining the configuration of a mitoplast membrane similar to that of the inner membrane of intact mitochondria under their swelling in hypotonic conditions. The prospects of using AFM to study the configuration of the surface of the mitochondrial inner membrane and other biological membrane systems were demonstrated.
KeywordsOsmotic Shock Small Angle Neutron Scattering Hypotonic Medium Electron Microscopy Photo Hypotonic Condition
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- 7.M. Yokokawa, K. Takeyasu, and S. H. Yoshimura, “Mechanical Properties of Plasma Membrane and Nuclear Envelope Measured by Scanning Probe Microscope,” J. Microsc. (Oxford, UK) 232, 82–90.Google Scholar
- 14.I. P. Krasinskaya, I. S. Litvinov, S. D. Zakharov, L. E. Bakeeva, and L. S. Yaguzhinsky, “Two Qualitatively Different Structural-Functional States of Mitochondria,” Biokhimiya (Moscow) 54, 1550–1556 (1989).Google Scholar
- 15.I. P. Krasinskaya, V. N. Marshanskii, S. F. Dragunova, and L. S. Yaguzhinskii, “Synchronization of the Functions of Enzymes of the Respiratory Chain and Adenosine Triphosphate Synthetase of Energized Mitochondria,” Biokhimiya (Moscow) 49, 87–92 (1984).Google Scholar
- 21.T. N. Murugova, V. I. Gordeliy, A. I. Kuklin, Yu. S. Kovalev, V. I. Yurkov, A. Nurenberg, A. Kh. Islamov, and L. S. Yaguzhinskii, “Detection of New Double-Membrane Structures in Native Mitochondria by the Method of Small-Angle Neutron Scattering,” Biofizika 51(6), 1001–1007 (2006) [Biophysics 51 (6), 882–886 (2006)].PubMedGoogle Scholar