Abstract
The effect of single-wall carbon nanotubes (carbon SWNTs) on bacterial cells Escherichia coli K12 TG1 with cloned lux-operon of natural marine bacteria Photobacterium leiognathi was studied. Using atomic force microscopy (AFM), bacterial cell morphological changes were revealed and a cell viability decrease controlled by the number of colony-forming units was registered. It was shown that prior to this we can observe changes in the intensity of oxygen consumption and bacterial luminescence intensity. This lets us recommend a well-known and easy-to-use bioluminescent test for the initial testing of nanomaterial toxicity using living bacteria cultures as well as lyophilized Ecolum biosensors.
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Original Russian Text © A.P. Zarubina, E.P. Lukashev, L.I. Deev, I.M Parkhomenko, A.B. Rubin, 2009, published in Rossiiskie nanotekhnologii, 2009, Vol. 4, Nos. 11–12.
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Zarubina, A.P., Lukashev, E.P., Deev, L.I. et al. Biotesting the biological effects of single-wall carbon nanotubes using bioluminescent bacteria test-system. Nanotechnol Russia 4, 871–875 (2009). https://doi.org/10.1134/S1995078009110159
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DOI: https://doi.org/10.1134/S1995078009110159