Abstract
The correlation was studied between the activity of tyrosinase electrodes engineered by layer-by-layer deposition technology and the number of PDDA/tyrosinase layers (where PDDA stands for poly(diallyldimethylammonium chloride)). The last deposited layer is the active one regardless of the number of layers. Atomic force microscopy (AFM) characterization of the topology of (PDDA/tyrosinase)1 and (PDDA/tyrosinase)2 films showed that these films have identical structures. The operational stability of tyrosinase biosensors coated with (PDDA/tyrosinase)1 films was examined. Various methods for stabilizing tyrosinase biosensors were tested. Crosslinking with glutaraldehyde (GA) improved 2.5-fold the operational stability of (PDDA/tyrosinase)1 films.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Y. Lvov, G. Decher, and H. Möhwald, “Assembly, Structural Characterization, and Thermal Behavior of Layer-by-Layer Deposited Ultrathin Films of Poly(vinylsulfate) and Poly(allylamine),” Langmuir 9, 481 (1993).
G. Decher, B. Lehr, K. Lowack, et al., “New Nanocomposite Films for Biosensors: Layer-by-Layer Adsorbed Films of Polyelectrolytes, Proteins, or DNA,” Biosens. Bioelectron. 9, 677 (1994).
J. Anzai, Y. Kobayashi, Y. Suzuki, et al., “Enzyme Sensors Prepared by Layer-by-Layer Deposition of Enzymes on a Platinum Electrode through Avidin-Biotin Interaction,” Sens. Actuators, B 52, 3 (1998).
N. Ferreyra, L. Coche-Guerente, and P. Labbe, “Construction of Layer-by-Layer Self-Assemblies of Glucose Oxidase and Cationic Polyelectrolyte onto Glassy Carbon Electrodes and Electrochemical Study of the Redox-Mediated Enzymatic Activity,” Electrochim. Acta 49, 477 (2004).
Y. Kobayashi, T. Hoshi, and J. Anzai, “Glucose and Lactate Biosensors Prepared by a Layer-by-Layer Deposition of Concanavalin A and Mannose-Labeled Enzymes: Electrochemical Response in the Presence of Electron Mediators,” Chem. Pharm. Bull. 49, 755 (2001).
D. Trau and R. Renneberg, “Encapsulation of Glucose Oxidase Microparticles within a Nanoscale Layer-by-Layer Film: Immobilization and Biosensor Applications,” Biosens. Bioelectron. 18, 1491 (2003).
W. Li, Z. Wang, Ch. Sun, et al., “Fabrication of Multilayer Films Containing Horseradish Peroxidase and Polycation-Bearing Os Complex by Means of Electrostatic Layer-by-Layer Adsorption and Its Application As a Hydrogen Peroxide Sensor,” Anal. Chim. Acta 418, 225 (2000).
M. K. Ram, P. Bertoncello, H. Ding, et al., “Cholesterol Biosensors Prepared by Layer-By-Layer Technique,” Biosens. Bioelectron. 16, 849 (2001).
J. Wang, G. Liu, and Y. Lin, “Amperometric Choline Biosensor Fabricated through Electrostatic Assembly of Bienzyme/Polyelectrolyte Hybrid Layers on Carbon Nanotubes,” Analyst (Cambridge, UK) 131, 477 (2006).
G. Liw and Y. Lin, “Biosensor Based on Self-Assembling Acetylcholinesterase on Carbon Nanotubes for Flow Injection/Amperometric Detection of Organophosphate Pesticides and Nerve Agents,” Anal. Chem. 78, 835 (2006).
G. V. Dubacheva, L. G. Sokolovskaya, L. V. Sigolaeva, et al., “Tyrosinase Biosensors Based on Nanostructured Films of Polyelectrolytes,” Sens. Sist. 20(4), 336 (2006).
S. Campuzano, B. Serra, M. Pedrero, et al., “Amperometric Flow-Injection Determination of Phenolic Compounds at Self-Assembled Monolayer-Based Tyrosinase Biosensors,” Anal. Chim. Acta 494, 187 (2003).
L. Coche-Guerente, P. Labbe, and V. Mengeaud, “Amplification of Amperometric Biosensor Responses by Electrochemical Substrate Recycling: 3. Theoretical and Experimental Study of the Phenol-Polyphenol Oxidase System Immobilized in Laponite Hydrogels and Layer-by-Layer Self-Assembled Structures,” Anal. Chem. 73, 3206 (2001).
G. V. Dubacheva, M. V. Porus, L. G. Sokolovskaya, et al., “Nanostructured Films of Polyelectrolytes as the Basis for the Design of Highly Sensitive Tyrosinase Biosensors,” Ross. Nanotechnol. 2(1–2), 154 (2007).
D. R. Tur, A. S. Peregudov, L. I. Komarova, et al., “Heterogeneous Synthesis of Water-Soluble Polydiamine-phosphazenes,” in Abstracts of Papers of the III All-Russian Kargin Conference “Polymers-2004,” Moscow, Russia, January 27–February 1, 2004 (Moscow State University, Moscow, 2004), Vol. 1, p. 148 [in Russian].
E. G. Evtushenko, I. N. Kurochkin, E. A. Dontsova, et al., “Nanometer-Sized Films of Antibodies Based on Polyelectrolytes for Purposes of Highly Sensitive Immunodiagnostics,” Ross. Nanotechnol. 2(1–2), 145 (2007).
A. Guerrieri, L. Monaci, M. Quinto, and F. Palmisano, “A Disposable Amperometric Biosensor for Rapid Screening of Anticholinesterase Activity in Soil Extracts,” Analyst (Cambridge, UK) 127, 5 (2002).
G. F. Khan and W. Wernet, “A Highly Sensitive Amperometric Creatinine Sensor,” Anal. Chim. Acta 351, 151 (1997).
L. Mao and K. Yamamoto, “Amperometric On-Line Sensor for Continuous Measurement of Hypoxanthine Based on Osmium-Polyvinylpyridine Gel Polymer and Xanthine Oxidase Bienzyme Modified Glassy Carbon Electrode,” Anal. Chim. Acta 415, 143 (2000).
T. Woedtke, W.-D. Julich, V. Hartmann, et al., “Sterilization of Enzyme Glucose Sensors: Problems and Concepts,” Biosens. Bioelectron. 17, 373 (2002).
H. Yamato, T. Koshiba, M. Ohwa, et al., “A New Method for Dispersing Palladium Microparticles in Conducting Polymer Films and Its Application to Biosensors,” Synth. Met. 87, 231 (1997).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © G.V. Dubacheva, M.V. Porus, L.V. Sigolaeva, D.V. Pergushov, D.R. Tur, V.S. Papkov, A.B. Zezin, A.A. Yaroslavov, A.V. Eremenko, I.N. Kurochkin, S.D. Varfolomeev, 2008, published in Rossiiskie nanotekhnologii, 2008, Vol. 3, Nos. 3–4.
Rights and permissions
About this article
Cite this article
Dubacheva, G.V., Porus, M.V., Sigolaeva, L.V. et al. Nanostructured polyelectrolyte films for engineering highly sensitive tyrosinase biosensors: Specifics of enzyme-polyelectrolyte structures. Nanotechnol Russia 3, 221–227 (2008). https://doi.org/10.1134/S1995078008030099
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1995078008030099