Russian Journal of Physical Chemistry B

, Volume 11, Issue 4, pp 663–665 | Cite as

Ammonium perchlorate detection in natural environments using specific lux biosensors

  • V. P. Balabanov
  • S. A. Khrulnova
  • V. Yu. Kotova
  • G. B. ZavilgelskyEmail author
Chemical Physics of Ecological Processes


A procedure involving specific lux biosensors has been applied to rapid detection of environmental ammonium perchlorate (AP) under both laboratory and field conditions. The procedure is based on evaluating the influence of AP on the level of bioluminescence of lux biosensors. Two lux biosensors—Escherichia coli MG1655 katG::kan (pKatG-lux) and E. coli MG1655 (pSoxS-lux)—have been employed in testing the ability of AP to cause an oxidative stress associated with the appearance of hydrogen peroxide and superoxide anion radicals in the cell. The lux biosensors designed contain hybrid plasmids with appropriate regulatory DNA regions transcriptionally fused with the bacterial luciferase lux genes as reporter genes. AP at concentrations of 5–50 mmol/L exerts an effect on the induction of luminescence of the lux biosensors with the PkatG and PsoxS promoters. The intensity of the bioluminescence of the cells that contain the pKatG-lux and pSoxS-lux plasmids and have been treated with AP is 5–10 higher than the intensity of the bioluminescence of the untreated cells. Therefore, AP induces an oxidative stress in the bacterial cells through the formation of reactive oxygen species, namely, hydrogen peroxide and the superoxide anion radical. The high sensitivity and specificity of the lux biosensors makes them usable in AP detection in the environment.


lux biosensors ammonium perchlorate bioluminescence 


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  1. 1.
    C. A. Vollmer and T. K. van Dyk, Adv. Microb. Physiol. 49, 131 (2004).CrossRefGoogle Scholar
  2. 2.
    L. Galluzzi and M. Karp, Comb. Chem. High Thorough-put Screen 9, 501 (2006).CrossRefGoogle Scholar
  3. 3.
    M. Woutersen, S. Belkin, B. Brouwer, et al., Anal. Bioanal. Chem. 400, 914 (2011).CrossRefGoogle Scholar
  4. 4.
    V. Yu. Kotova, I. V. Manukhov, and G. B. Zavilgelskii, Appl. Biochem. Microbiol. 46, 781 (2010).CrossRefGoogle Scholar
  5. 5.
    G. B. Zavil’gel’skii, V. Yu. Kotova, and A. S. Mironov, Russ. J. Phys. Chem. B 9, 454 (2015).CrossRefGoogle Scholar
  6. 6.
    T. K. van Dyk and R. A. Rosson, Methods in Molecular Biology, Vol. 102: Bioluminescence Methods and Protocols, Ed. by R. A. Rosson (Humana, Totowa, NJ, 1998), p. 85.Google Scholar
  7. 7.
    J. Sambrook and D. W. Russell, Molecular Cloning: A Laboratory Manual, 3rd ed. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 2001).Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • V. P. Balabanov
    • 1
  • S. A. Khrulnova
    • 1
    • 2
  • V. Yu. Kotova
    • 1
  • G. B. Zavilgelsky
    • 1
    Email author
  1. 1.State Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia
  2. 2.Scientific Centre for HaematologyMinistry of Health of the Russian FederationMoscowRussia

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