Russian Journal of Physical Chemistry B

, Volume 11, Issue 4, pp 587–593 | Cite as

Effect of a preliminary physicochemical treatment of bituminous crusts resulting from oil pollution on the bioremediation of upland swamps with oil decomposers

  • S. N. GaydamakaEmail author
  • V. P. Murygina
  • M. A. Gladchenko
Chemical Physics of Ecological Processes


Physicochemical preprocessing of a bituminous crust resulting from oil pollution by various reagents capable of hydrolyzing its structure, followed by aerobic degradation of hydrocarbons (HC) with the Rhoder bacterial oil-degrading preparation, was carried out. The crust was hydrolyzed with hydrogen dioxide, calcium hydroxide, and alkaline hydrolysate of crop production waste in concentrations from 2 to 20%. The best results in the destruction of the bituminous crust were obtained for processing the upper layer (0−2 cm) of the soil with calcium hydroxide, which resulted in an increase of the humidity of the hydrolyzed layer from 24 to 77%. The destruction of the bituminous crust with chemical agents made it possible to increase the effectiveness of biodegradation of HC from 9.97 to 26.40% in the upper soil layer and from 0.00 to 48.20% in the bottom soil layer. The kinetic characteristics of biodegradation after preprocessing, namely the rate constants of degradation and half-lives of HC were determined, the maximum values of which were found to be 0.015 day−1 and 47 days and 0.032 day−1 and 22 days for the upper and bottom soil layers, respectively.


swamp oil bituminous crust bioremediation microorganisms oil-degrading preparation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    V. A. Kryukov, European Energy and Climate Security, Vol. 31 of Lecture Notes in Energy (Springer, Switzerland, 2016), p. 81.CrossRefGoogle Scholar
  2. 2.
    A. E. Kuznetsov, N. B. Gradova, S. V. Lushnikov, et al., Applied Ecobiotechnology (Binom, Moscow, 2010) [in Russian].Google Scholar
  3. 3.
    Report on Ecological Situation in Khanty-Mansi Autonomous Area–Yugra in 2015, Khanty-Mansiysk, 2015. Scholar
  4. 4.
    A. A. Zubaidullin, in Collection of Scientific Works of Surgut University (Defis, Surgut, 2003), No. 6, p. 129 [in Russian].Google Scholar
  5. 5.
    Yu. A. Vershinin and A. A. Zubaidullin, Vestn. Nizhnevartov. Guman. Univ., No. 1, 53 (2009).Google Scholar
  6. 6.
    A. Singh, N. Parmar, and R. C. Kuhad, Bioaugmentation, Biostimulation and Biocontrol, Book Soil Biology (Springer, Berlin, Heidelberg, 2011).CrossRefGoogle Scholar
  7. 7.
    V. P. Murygina, S. N. Gaydamaka, M. A. Gladchenko, and A. A. Zubaydullin, Int. Biodeteriorat. Biodegrad. 114, 150 (2016).CrossRefGoogle Scholar
  8. 8.
    A. I. Albulov, A. Ya. Samuilenko, M. A. Frolova, et al., Izv. Samar. Nauch. Tsentra RAN 15, 1545 (2013).Google Scholar
  9. 9.
    I. P. Kalinkin, New Handbook of Chemist and Technologics, Analytical Chemistry (Mir Sem’ya, St. Petersburg, 2002), Part 1 [in Russian].Google Scholar
  10. 10.
    L. A. Vorob’eva, Chemical Analysis of Soils (Mosk. Gos. Univ., Moscow, 1998) [in Russian].Google Scholar
  11. 11.
    V. G. Mineev, Practical Guide on Agrochemistry (Mosk. Gos. Univ., Moscow, 2001) [in Russian].Google Scholar
  12. 12.
    Yu. S. Drugov, I. G. Zenkevich, and A. A. Rodin, Gas Chromatographical Identification of Pollution of Air, Waters, Soils and Biological Media (Binom, Moscow, 2005) [in Russian].Google Scholar
  13. 13.
    B. S. Bokshtein and M. I. Mendelev, Short Course of Physical Chemistry, The School-Book, 2nd ed. (CheRo, Moscow, 2001), p. 232 [in Russian].Google Scholar
  14. 14.
    A. I. Netrusov, Practical Guide on Microbiology (Akademiya, Moscow, 2005) [in Russian].Google Scholar
  15. 15.
    T. N. Nazina, E. P. Rozanova, S. S. Belyaev, and M. V. Ivanov, Preprint (Biol. Press Tsentr, Pushchino, 1988).Google Scholar
  16. 16.
    Yu. Sivkov, Indian J. Sci. Technol. 9 (14), 1 (2016).CrossRefGoogle Scholar
  17. 17.
    M. A. Gladchenko, S. N. Gaidamaka, V. P. Murygina, A. B. Lifshits, and P. G. Cherenkov, Russ. J. Phys. Chem. B 9, 429 (2015).CrossRefGoogle Scholar
  18. 18.
    V. Murygina, S. Gaidamaka, M. Iankevich, and A. Tumasyanz, in Proceedings of the International Symposium on Environmental Science and Technology, Dongguan, Guangdong, China, 2011, Vol. 3, p. 791.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • S. N. Gaydamaka
    • 1
    Email author
  • V. P. Murygina
    • 1
  • M. A. Gladchenko
    • 1
  1. 1.Department of Chemical EnzymologyMoscow State UniversityMoscowRussia

Personalised recommendations