Russian Journal of Physical Chemistry B

, Volume 9, Issue 7, pp 1005–1010 | Cite as

Xenogenic bone matrix treated with supercritical carbon dioxide as a potential osteoplastic material

  • D. Yu. Zalepugin
  • V. V. Zaitzev
  • N. A. Tilkunova
  • I. V. Chernyshova
  • I. I. Selezneva
  • Yu. A. Nikonova
  • M. I. Vlasov


A multistage environmentally safe method for processing xenogenic bone matrix using suband supercritical media has been developed. This method provides fast matrix delipidization in a medium of supercritical carbon dioxide and deproteinization by treatment with a 3% hydrogen peroxide solution followed by washing with subcritical water for removal of polar substances. The final treatment with supercritical carbon dioxide serves to remove residual nonpolar components. A method to control the porosity of xenogenic bone matrix at different stages of its purification has been suggested. Preliminary data indicate that a cellular test system based on an allogenic osteoid cell line Th-1 could be used for evaluation of primary adhesion and proliferation of cells on the surface of purified bone matrix.


supercritical extraction porosity carbon dioxide xenogenic matrix osteoplastic materials 


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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • D. Yu. Zalepugin
    • 1
  • V. V. Zaitzev
    • 2
  • N. A. Tilkunova
    • 1
  • I. V. Chernyshova
    • 1
  • I. I. Selezneva
    • 3
  • Yu. A. Nikonova
    • 3
  • M. I. Vlasov
    • 1
  1. 1.Federal State Unitary Enterprise “State Plant of Medicinal Drugs” (GosZMP)MoscowRussia
  2. 2.Federal State Institution of Science “Priorov Central Institute of Traumatology and Orthopaedics”MoscowRussia
  3. 3.Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics”Russian Academy of SciencesPushchino, Moscow oblastRussia

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