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A New Human Uveal Melanoma Cell Line: Melanin Production and Molecular Markers for Targeted Therapy

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Abstract

A new human uveal melanoma (UM) cell line uMel1 was established by mechanical disintegration of a tumor fragment. uMel1 cells had a stellate dendrite-like shape, contained a lot of brown melanin pigment, and had a low mitotic index. Optimization of cultivation conditions led to an increase in the rate of cell proliferation and was accompanied by the loss of brown pigment. Since the melanin precursor is L-dihydroxyphenylalanine (L-DOPA), the authors analyzed the cultivation of uMel1 cells in the presence of L-DOPA. When L-DOPA was used at a concentration of 20 μg/mL, causing a decrease in cell viability by no more than 10%, melanocytes uMel1 synthesized melanin. It can be concluded that cultivation in the presence of L-DOPA provides the phenotype of melanin-containing melanocytes of uMel1 personal culture under conditions of long-term cultivation. Analysis of cell adhesion molecules N-cadherin (N-cad), E-cadherin (E-cad), and Mel-CAM, as well as receptors of the epidermal growth factor (ErbB) family by flow cytometry, showed that uMel1 cells have a phenotype of N-cad–/E-cad–/Mel-CAM+/HER2low/HER3low, and can be used for the study of targeted drugs to Mel-CAM, HER2 and HER3.

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Funding

The research was supported by the Russian Science Foundation (project no. 23-14-00285) (establishment of the cell culture and characterization of molecular markers) and the budget funding project of the Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences (no. 121030200173-6) (experiments with L-DOPA).

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    M. V. Zhilnikova, D. D. Novak, O. S. Troitskaya, A. A. Nushtaeva, M. M. Biryukov, S. P. Zvereva, M. E. Varlamov, V. V. Koval, O. M. Stanishevskaya, D. V. Chernikh, V. V. Atamanov & O. A. Koval

  2. Novosibirsk State University, 630090, Novosibirsk, Russia

    M. V. Zhilnikova, D. D. Novak, M. M. Biryukov & O. A. Koval

  3. Novosibirsk Branch, Fedorov National Medical Research Center Interindustry Scientific and Technical Complex Eye Microsurgery, 630096, Novosibirsk, Russia

    O. M. Stanishevskaya, D. V. Chernikh, N. V. Kononova & V. V. Atamanov

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  1. M. V. Zhilnikova
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Correspondence to O. A. Koval.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

All studies involving humans were conducted in accordance with the principles of biomedical ethics as outlined in the 1964 Declaration of Helsinki and its later amendments. They were also approved by the ethical committee of the Novosibirsk Branch of Fedorov National Medical Research Center Interdisciplinary Scientific and Technical Complex Eye Microsurgery. Informed consent to participate in the study was obtained from all study participants.

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Abbreviations: a/a, antibiotic-antimycotic; UM, uveal melanoma; CAR, chimeric antigen receptor; E-cad, cadherin E; N‑cad, cadherin N; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor; HER2, HER3, human epidermal growth factor receptor 2, 3; L-DOPA, L-dihydroxyphenylalanine; Mel-CAM, surface glycoprotein, melanoma cell adhesion molecule; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide; PBS, phosphate buffer saline; VEGF-A, vascular endothelial growth factor A.

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Zhilnikova, M.V., Novak, D.D., Troitskaya, O.S. et al. A New Human Uveal Melanoma Cell Line: Melanin Production and Molecular Markers for Targeted Therapy. Biochem. Moscow Suppl. Ser. B 17, 165–171 (2023). https://doi.org/10.1134/S1990750823600607

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