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Analysis of Phosphatidylcholines Alterations in Human Glioblastomas Ex Vivo

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract—

The analysis of data obtained using ambient mass spectrometric profiling of clinical samples of primary and secondary human glioblastomas ex vivo, revealed changes in the lipid composition of tissues accompanying the development of grade IV glial tumors (according to the WHO classification). It has been shown that both primary and secondary (developed from astrocytoma) tumors are enriched with mono- and di-unsaturated phosphatidylcholines (PC 26:1, 30:2, 32:1, 32:2, 34:1, 34:2). At the same time, the content of saturated and polyunsaturated phosphatidylcholines and phosphatidylethanolamines decreases. These changes are associated both with altered availability of polyunsaturated fatty acids and activation of de novo synthesis and beta-oxidation of fatty acids under conditions of oxygen starvation in the tumor body.

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Funding

The research was supported by the Ministry of Science and Higher Education of the Russian Federation (agreement no. 075-00337-20-02, project no. 0714-2020-0006). The research used the equipment of Shared Research Facilities of the Semenov Federal Research Center for Chemical Physics RAS.

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Authors and Affiliations

  1. Skolkovo Institute of Science and Technology, Territory of “Skolkovo” Innovation Center, Bolshoy blvd. 30, bldg. 1, 121205, Moscow, Russia

    S. I. Pekov & E. N. Nikolaev

  2. Moscow Institute of Physics and Technology (National Research University), Institutsky per. 9, 141701, Dolgoprudny, Moscow oblast, Russia

    S. I. Pekov, A. A. Sorokin, A. A. Kuzin, D. S. Bormotov, A. S. Shivalin, V. A. Shurkhay & I. A. Popov

  3. Semenov Federal Center of Chemical Physic of RAS, ul. Kosygina 4, bldg. 1, 119991, Moscow, Russia

    K. V. Bocharov

  4. Burdenko National Medical Research Center of Neurosurgery, ul. 4ya Tverskaya-Yamskaya 16, 125047, Moscow, Russia

    V. A. Shurkhay & A. A. Potapov

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  1. S. I. Pekov
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  2. A. A. Sorokin
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  3. A. A. Kuzin
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  4. K. V. Bocharov
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  5. D. S. Bormotov
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  6. A. S. Shivalin
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  7. V. A. Shurkhay
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  8. A. A. Potapov
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  9. E. N. Nikolaev
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  10. I. A. Popov
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Corresponding author

Correspondence to I. A. Popov.

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The authors declare that they have no conflicts of interest. The study was conducted in accordance with the recommendations of the Ethical Committee of the Burdenko National Medical Research Center of Neurosurgery (the Order 40 of April 12, 2016 as amended by the Order 131 of July 17, 2018). Prior to surgery, all patients gave informed consent for the use the excised tissue samples in scientific research in accordance with the legislation and the requirements of the Ethics Committee. The study was conducted in accordance with the Declaration of Helsinki, revised in 2013. All protocols and procedures were carried out in accordance with the relevant guidelines and rules approved by the Supervisory Board of the Burdenko National Medical Research Center of Neurosurgery.

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Translated by A. Medvedev

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Pekov, S.I., Sorokin, A.A., Kuzin, A.A. et al. Analysis of Phosphatidylcholines Alterations in Human Glioblastomas Ex Vivo. Biochem. Moscow Suppl. Ser. B 15, 241–247 (2021). https://doi.org/10.1134/S1990750821030070

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  • DOI: https://doi.org/10.1134/S1990750821030070

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