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Expression of Sperm-Specific Glyceraldehyde-3-Phosphate Dehydrogenase in Melanoma Cells Changes Their Energy Metabolism

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Abstract—

The somatic isoform of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) is involved in such crucial pathways for cancer cells development as induction of apoptosis and regulation of glycolysis. At the same time, sperm-specific isoform (GAPDHS) does not exhibit all the same functions as the somatic enzyme. The expression of sperm-specific GAPDH without N-terminal domain in some melanoma cells along with somatic isoenzyme, shown in our previous work, has led to the proposal of this unusual enzyme’s possible role in regulation of cancer cells glycolysis. In this study we have tested production of GAPDHS in 13 additional melanoma cell lines by immunoblotting. We have also gathered data on energy metabolism in 5 selected cell lines by evaluating glucose uptake and lactate production in different conditions. We have demonstrated that in standard cultivation media glucose uptake by MelP cells, producing substantial amounts of GAPDHS protein was higher than in MelKor cells, producing lesser amounts of GAPDHS. All other analyzed cell lines that do not produce GAPDHS (MelMS, MelSi and Malme3M) had even a lower glucose uptake rate.

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REFERENCES

  1. Muronetz, V.I., Melnikova, A.K., Barinova, K.V., and Schmalhausen, E.V., Biochemistry (Moscow), 2019, vol. 84, p. 1268–1279. https://doi.org/10.1134/s0006297919110051

    Article  CAS  PubMed  Google Scholar 

  2. Pistritto, G., Trisciuoglio, D., Ceci, C., Garufi, A., and D’Orazi, G., Aging, 2016, vol. 8, pp. 603–619. https://doi.org/10.18632/aging.100934

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Akram, M., J. Cancer Education, 2013, vol. 28, pp. 454–457. https://doi.org/10.1007/s13187-013-0486-9

    Article  CAS  Google Scholar 

  4. Sirover, M.A., Biochim. Biophys. Acta, 1999, vol. 1432, pp. 159–184. https://doi.org/10.1016/j.bbagen.2011.05.010

    Article  CAS  PubMed  Google Scholar 

  5. Schmalhausen, E.V. and Muronetz, V.I., Biosci. Rep., 1997, vol. 17, pp. 521–527. https://doi.org/10.1023/a:1027356106330

    Article  CAS  PubMed  Google Scholar 

  6. Schmalhausen, E.V., Nagradova, N.K., Boschi-Muller, S., Branlant, G., and Muronetz, V.I., FEBS Letts, 1999, vol. 452, pp. 219–222. https://doi.org/10.1016/s0014-5793(99)00627-4

    Article  CAS  Google Scholar 

  7. Warburg, O., Science, 1956, vol. 123, pp. 309–314. https://doi.org/10.1126/science.123.3191.309

    Article  CAS  PubMed  Google Scholar 

  8. Tompa, P. and Batke J., Biochem. Int., 1990, vol. 20, pp. 487–494.

    CAS  PubMed  Google Scholar 

  9. Fokina, K.V., Dainyak, M.B., Nagradova, N.K., and Muronetz, V.I., Arch. Biochem. Biophys., 1997, vol. 345, pp. 185–192. https://doi.org/10.1006/abbi.1997.0222

    Article  CAS  PubMed  Google Scholar 

  10. Walsh, J.L., Keith, T.J., and Knull, H.R., Biochim. Biophys. Acta, 1989, vol. 999, pp. 64–70. https://doi.org/10.1016/0167-4838(89)90031-9

    Article  CAS  PubMed  Google Scholar 

  11. Muronetz, V.I., Wang, Z.X., Keith, T.J., Knull, H.R., and Srivastava, D.K., Arch. Biochem. Biophys., 1994, vol. 313, pp. 253–260. https://doi.org/10.1006/abbi.1994.1385

    Article  CAS  PubMed  Google Scholar 

  12. Patra, S., Ghosh, S., Bera, S., Roy, A., Ray, S., and Ray, M., Biochemistry (Moscow), 2009, vol. 74, pp. 717–727. https://doi.org/10.1134/s0006297909070037

    Article  CAS  PubMed  Google Scholar 

  13. Sevostyanova, I.A., Kulikova, K.V., Kuravsky, M.L., Schmalhausen, E.V., and Muronetz, V.I., Biochem. Biophys. Res. Commun., 2012, vol. 427, pp. 649–653. https://doi.org/10.1016/j.bbrc.2012.09.115

    Article  CAS  PubMed  Google Scholar 

  14. Kuravsky, M.L., Aleshin, V.V., Frishman, D., and Muronetz, V.I., BMC Evolutionary Biology, 2011, vol. 11, 160. https://doi.org/10.1186/1471-2148-11-16

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Elkina, Y.L., Kuravsky, M.L., El’darov, M.A., Stogov, S.V., Muronetz, V.I., and Schmalhausen, E.V., Biochim. Biophys. Acta, 2010, vol. 1804, pp. 2207–2212. https://doi.org/10.1016/j.bbapap.2010.09.002

    Article  CAS  PubMed  Google Scholar 

  16. Scopes, R.K. and Stoter, A., Methods Enzymol., 1982, vol. 90, pp. 479–490. https://doi.org/10.1016/S0076-6879(82)90175-6

    Article  CAS  PubMed  Google Scholar 

  17. Mikhaylova, I.N., Kovalevsky, D.A., Morozova, L.F., Golubeva, V.A., Cheremushkin, E.A., Luka-shina, M.I., et al., Melanoma Research, 2008, vol. 18, pp. 303–313. https://doi.org/10.1097/CMR.0b013e32830e391d

    Article  CAS  PubMed  Google Scholar 

  18. Danshina, P.V., Schmalhausen, E.V., Avetisyan, A.V., and Muronetz, V.I., IUBMB Life, 2001, vol. 51, pp. 309–314. https://doi.org/10.1080/152165401317190824

    Article  CAS  PubMed  Google Scholar 

  19. Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685. https://doi.org/10.1038/227680a0

    Article  CAS  Google Scholar 

  20. Kuravsky, M.L., Schmalhausen, E.V., Pozdnyakova, N.V., and Muronetz, V.I., Anal. Biochem., 2012, vol. 426, pp. 47–53. https://doi.org/10.1016/j.ab.2012.03.026

    Article  CAS  PubMed  Google Scholar 

  21. Grigorieva, J.A., Dainiak, M.B., Katrukha, A.G., and Muronetz, V.I., Arch. Biochem. Biophys., 1999, vol. 369, pp. 252–260. https://doi.org/10.1006/abbi.1999.1341

    Article  CAS  PubMed  Google Scholar 

  22. Hoek, K.S., Schlegel, N.C., Eichhoff, O.M., Widmer, D.S., Praetorius, C., Einarsson, S.O., et al., Pigment Cell Melanoma Research, 2008, vol. 21, pp. 665–676. https://doi.org/10.1111/j.1755-148X.2008.00505.x

    Article  CAS  PubMed  Google Scholar 

  23. Lekmine, F., Chang, C.K., Sethakorn, N., Das Gupta, T.K., and Salti, G.I., Biochem. Biophys. Res. Commun., 2007, vol. 354, pp. 830–835. https://doi.org/10.1016/j.bbrc.2007.01.075

    Article  CAS  PubMed  Google Scholar 

  24. Sajnani, K., Islam, F., Smith, R.A., Gopalan, V., and Lam, A.K.-Y., Biochimie, 2017, vol. 135, pp. 164–172. https://doi.org/10.1016/j.biochi.2017.02.008

    Article  CAS  PubMed  Google Scholar 

  25. Tsubaki, M. and Yoshikawa, S., Biochemistry, 1993, vol. 32, pp. 174–182. https://doi.org/10.1021/bi00052a023

    Article  CAS  PubMed  Google Scholar 

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Funding

This work was supported by the Russian Foundation for Basic Research (project no. 18-34-00132_mol_a).

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Authors and Affiliations

  1. Faculty of Bioengineering and Bioinformatics, Moscow State University, 119234, Moscow, Russia

    A. K. Melnikova & V. I. Muronetz

  2. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119234, Moscow, Russia

    M. L. Kuravsky, I. A. Sevostyanova & V. I. Muronetz

  3. Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    K. V. Kulikova

Authors
  1. A. K. Melnikova
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  2. M. L. Kuravsky
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  3. K. V. Kulikova
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  4. I. A. Sevostyanova
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  5. V. I. Muronetz
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Correspondence to A. K. Melnikova.

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This work does not contain any research using humans and animals as research objects.

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Translated by A. Medvedev

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Melnikova, A.K., Kuravsky, M.L., Kulikova, K.V. et al. Expression of Sperm-Specific Glyceraldehyde-3-Phosphate Dehydrogenase in Melanoma Cells Changes Their Energy Metabolism. Biochem. Moscow Suppl. Ser. B 15, 105–110 (2021). https://doi.org/10.1134/S1990750821020062

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  • DOI: https://doi.org/10.1134/S1990750821020062

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