Skip to main content
SpringerLink
Log in

Proteins and Immunohistochemical Markers of Breast Diseases

  • Published:
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract—

Malignant and benign diseases of the breast have been compared in terms of 8 proteins: histidine-rich glycoprotein (HRG), mucin 1 (MUC1), plasminogen activator inhibitor 1 (PAI-1), heat shock protein 90αA1 (HSP90αA1), E-cadherin (CDH1), estrogen receptor α (ERα), progesterone receptor (PGR) and interleukin 12 (IL-12). Their concentrations were determined in the supernatants of immunocompetent blood cells and breast biopsy samples during spontaneous and polyclonal activator induced production and after exposure of biopsy samples to the differentiation factor HLDF. In addition, the indices of the effect of the polyclonal activator and HLDF on the production of these proteins were calculated and the correlation relationships of the above indicators with the expression of markers of the epithelial-mesenchymal transition (EMT), including type II collagen (CII), β-1 integrin (CD29), and cadherin-E (CDH1), were studied. Statistically significant differences were found in the HRG concentration in the supernatant of immunocompetent blood cells, IL-12 during spontaneous production by biopsy samples, PGR in the case of biopsy specimens induced by a polyclonal activator, CDH1 and IL-12 exposure of biopsy samples to HLDF. According to the indices of the influence of the polyclonal activator and HLDF, statistically significant differences were found in the case of CDH1 production. Comparison of the EMT markers in biopsy samples of invasive carcinoma of no special type (IC-NST) and benign diseases of the breast revealed statistically significant differences in the rates of expression of CD29, while there were no differences in expression of CDH1 and CII. This suggests the presence of cell atypia in biopsy samples of benign breast diseases and is confirmed by existing correlation between production of certain proteins and expression of the EMT markers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Torre, L.A., Bray, F., Siegel, R.L., Ferlay, J., Lortet-Tieulent, J., and Jemal, A., CA: A Cancer Journal For Clinicians, 2015, vol. 65, no. 2, pp. 87−108. https://doi.org/10.3322/caac.21262

    Article  Google Scholar 

  2. Zeidan, B.A., Townsend, P.A., Garbis, S.D., Copson, E., and Cutress, R.I., Surgeon, 2015, vol. 13, no. 5, pp. 271−278. https://doi.org/10.1016/j.surge.2014.12.003

    Article  PubMed  Google Scholar 

  3. Shukla, H.D., Mahmood, J., and Vujaskovic, Z., Cancer Lett., 2015, vol. 369, no. 1, pp. 28−36. https://doi.org/10.1016/j.canlet.2015.08.003

    Article  CAS  PubMed  Google Scholar 

  4. Kulasingam, V. and Diamandis, E.P., Nat. Clin. Pract. Oncol., 2008, vol. 5, no. 10, pp. 588−599. https://doi.org/10.1038/ncponc1187

    Article  CAS  PubMed  Google Scholar 

  5. Rolny, C., Mazzone, M., Tugues, S., Laoui, D., Johansson, I., Coulon, C., Squadrito, M.L., Segura, I., Li, X., Knevels, E., Costa, S., Vinckier, S., Dresselaer, T., Åkerud P., de Mol, M., Salomäki, H., Phillipson, M., Wyns, S., Larsson, E., Buysschaert, I., Botling, J., Himmelreich, U., van Ginderachter, J.A., de Palma, M., Dewerchin, M., Claesson-Welsh, L., and Carmeliet, P., Cancer Cell, 2011, vol. 19, no. 1, pp. 31−44. https://doi.org/10.1016/j.ccr.2010.11.009

    Article  CAS  PubMed  Google Scholar 

  6. Elissa, S., Matboli, M., and Shehata, H.H., Transl. Res., 2015, vol. 165, no. 3, pp. 417−427. https://doi.org/10.1016/j.trsl.2014.10.001

    Article  CAS  Google Scholar 

  7. Autenshlyus, A.I., Golovanova, A.V., Studenikina, A.A., Zhurakovskiy, I.P., Arhipov, S.A., Proskura, A.V., Vavilin, V.A., Lyakhovich, V.V., Brusentsov, I.I., and Sidorov, S.V., Dokl. Biochem. Biophys., 2019, vol. 484, no. 1, pp. 59−62. https://doi.org/10.1134/S1607672919010162

    Article  CAS  PubMed  Google Scholar 

  8. Huang, X., Sun, Q., Chen, C., Zhang, Y., Kang, X., Zhang, J.Y., Ma, D.W., Xia, L., Xu, L., Xu, X.Y., and Ren, B.H., Oncotarget, 2017, vol. 8, pp. 90315−90326. https://doi.org/10.18632/oncotarget.19861

  9. Witzel, I., Milde-Langosch, K., Schmidt, M., Karn, T., Becker, S., Wirtz, R., Rody, A., Laakmann, E., Schütze, D., Jänicke, F., and Müller, V., Onco Targets Ther., 2014, vol. 7, pp. 2205−2213. https://doi.org/10.2147/OTT.S65344

    Article  PubMed  PubMed Central  Google Scholar 

  10. Chen, B., Piel, W.H., Gui, L., Bruford, E., and Monteiro, A., Genomics, 2005, vol. 86, pp. 627−637. https://doi.org/10.1016/j.ygeno.2005.08.012

    Article  CAS  PubMed  Google Scholar 

  11. Zagouri, F., Sergentanis, T.N., Nonni, A., Papadimitriou, C.A., Michalopoulos, N.V., Domeyer, P., Theodoropoulos, G., Lazaris, A., Patsouris, E., Zogafos, E., Pazaiti, A., and Zografos, G.C., BMC Cancer, 2010, vol. 10, 353. https://doi.org/10.1186/1471-2407-10-353

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Zeng P., Sun S., Li R., Xiao Z.X., and Chen H., Int. J. Mol. Sci., 2019, vol. 20, no. 9, pii: E2223. https://doi.org/10.3390/ijms20092223

    Article  CAS  PubMed  Google Scholar 

  13. Wong, S.H.M., Fang, C.M., Chuah, L.H., Leong, C.O., and Ngai, S.C., Crit. Rev. Oncol. Hematol., 2018, vol. 121, pp. 11−22. https://doi.org/10.1016/j.critrevonc.2017.11.010

    Article  PubMed  Google Scholar 

  14. Pettersson, K. and Gustafsson, J.A., Annu. Rev. Physiol., 2001, vol. 63, pp. 165−192. https://doi.org/10.1146/annurev.physiol.63.1.165

    Article  CAS  PubMed  Google Scholar 

  15. Jafarzadeh, A., Minaee, K., Farsinejad, A.R., Nemati, M., Khosravimashizi, A., Daneshvar, H., Mohammadi, M.M., Sheikhi, A., and Ghaderi, A., Iran J. Basic. Med. Sci., 2015, vol. 18, no. 12, pp. 1189−1198.

    PubMed  PubMed Central  Google Scholar 

  16. Youssef, S.S., Mohammad, M.M., and Ezz-El-Arab, L.R., J. Med. Sci., 2015, vol. 3, no. 4, pp. 640−644. https://doi.org/10.3889/oamjms.2015.106

    Article  Google Scholar 

  17. Freeman, A.E. and Hoffman, R.M., Proc. Natl. Acad. Sci. USA, 1986, vol. 83, no. 8, pp. 2694−2698. https://doi.org/10.1073/pnas.83.8.2694

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Zhou, S., Sun, X., Yu, L., Zhou, R., Li, A., Li, M., and Yang, W.J., J. Cancer, 2018, vol. 9, no. 3, pp. 604−613. https://doi.org/10.7150/jca.19190

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Kostanyan, I.A., Astapova, M.V., Starovoytova, E.V., Dranitsina, S.M., and Lipkin, V.M., FEBS Lett., 1994, vol. 356, nos. 2-3, pp. 327−329.

    Article  CAS  PubMed  Google Scholar 

  20. dos Santos, P.B., Zanetti, J.S., Ribeiro-Silva, A., and Beltrão, E.I., Diagn. Pathol., 2012, vol. 7, 104. https://doi.org/10.1186/1746-1596-7-104

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. le Bras, G.F., Taubenslag, K.J., and Andl, C.D., Cell Adhesion Migration, 2012, vol. 6, no. 4, pp. 365−373. https://doi.org/10.4161/cam.21326

    Article  PubMed  PubMed Central  Google Scholar 

  22. Lou, P., Li, C., Shi, L., Xia, T.S., Zhou, W., Wu, J., Zhou, X., Li, X., Wang, Y., Wei, J.F., and Ding, Q., Oncotarget, 2017, vol. 8, no. 10, pp. 16387−16400. https://doi.org/10.18632/oncotarget.12016

    Article  PubMed  Google Scholar 

  23. rieto-García, E., Díaz-García, C.V., García-Ruiz, I., and Agulló-Ortuño, M.T., Med. Oncol., 2017, vol. 34, 122. 10.1007/s12032-017-0980-

    Article  CAS  PubMed  Google Scholar 

  24. Lal, I., Dittus, K., and Holmes, C.E., Breast Cancer Res., 2013, vol. 15, no. 4, 207.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Franchi, M., Masola, V., Bellin, G., Onisto, M., Karamanos, K.A., and Piperigkou, Z., J. Clin. Med., 2019, vol. 8, no. 2, 213. https://doi.org/10.3390/jcm8020213

    Article  CAS  PubMed Central  Google Scholar 

Download references

Funding

The study was performed within the framework of the State Assignment of the Ministry of Public Health of the Russian Federation (no. AAAA-A18-118030790008-7).

Author information

Authors and Affiliations

  1. Novosibirsk State Medical University, Krasnyi pr. 52, 630091, Novosibirsk, Russia

    A. I. Autenschlyus, A. V. Bernado, K. I. Davletova, S. A. Arkhipov, I. P. Zhurakovsky & E. S. Mikhailova

  2. Institute of Molecular Biology and Biophysics, Division of the Federal Research Center for Fundamental and Translational Medicine, ul. Timakova 2/12, 630117, Novosibirsk, Russia

    A. I. Autenschlyus, S. A. Arkhipov, I. P. Zhurakovsky, E. S. Mikhailova, A. V. Proskura & V. V. Lyakhovich

  3. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    A. P. Bogachuk & V. M. Lipkin

Authors
  1. A. I. Autenschlyus
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Bernado
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. I. Davletova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. A. Arkhipov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. P. Zhurakovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. S. Mikhailova
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. V. Proskura
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. P. Bogachuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. V. M. Lipkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. V. V. Lyakhovich
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. I. Autenschlyus.

Ethics declarations

COMPLIANCE WITH ETHICAL STANDARDS

All studies were performed in accordance with the Helsinki Declaration of the World Medical Association. All persons involved in this study gave informed consent to participate in this study and to use their blood and tumor biopsy samples for the research purposes. The Ethics Committee of the Institute of Molecular Biology and Biophysics, a Division of the Federal Research Center for Fundamental and Translational Medicine (Novosibirsk) gave permission for this study.

CONFLICT OF INTERESTS

The authors declare that they have no conflict of interests.

Additional information

Translated by A. Medvedev

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Autenschlyus, A.I., Bernado, A.V., Davletova, K.I. et al. Proteins and Immunohistochemical Markers of Breast Diseases. Biochem. Moscow Suppl. Ser. B 14, 306–311 (2020). https://doi.org/10.1134/S1990750820040022

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990750820040022

Keywords:

Search

Navigation