Abstract—
Exosome secretion has been demonstrated in the model of induced neuronal resistance to the toxic effect of glutamate (deprivation of trophic factors). Exosomes secretion occurring in the course of development of resistance during deprivation and at the first 24 h after preconditioning, as was shown by dot blot of extracellular fluid using anti-CD63 antibody. The autophagy inhibitor bafilomycin (0.01 µM) significantly reduced the quantity of the secreted exosomes at the stage of autophagy induction and during the first 24 h after induction. At the same time, inhibition of autophagy during trophic factor deprivation prevented the development of resistance, while inhibition of autophagy during the first 24 h after deprivation did not affect the development of resistance. We suggest that the long-term effects of preconditioning may be mediated by exosome secretion.
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The work was carried out the framework of the State Assignments “Multidisciplinary study of the molecular and genetic mechanisms of neuronal plasticity and neurodegeneration” (no. 0129-2014-0002, Institute of Higher Nervous Activity, RAS) and Research Center of Neurology no. 116 012 610 014 “Investigation of cellular and molecular mechanisms of the pathogenesis of neurodegenerative forms of cerebral pathology during their experimental modeling using modern cellular technologies.”
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Translated by A. Medvedev
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Yakovlev, A.A., Lyzhin, A.A., Aleksandrova, O.P. et al. Exosomes Secretion and Autophagy in Long-Term Protection of Neurons from Excitotoxic Damage. Biochem. Moscow Suppl. Ser. B 14, 1–5 (2020). https://doi.org/10.1134/S1990750820010151
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DOI: https://doi.org/10.1134/S1990750820010151