Abstract
Organophosphorus pesticides (OP) are used to protect crops from pests. Treatment of plants and animals with pesticides can be done during their growth or creation of conditions required for the long-shelf life of the agricultural products. Currently, many remedies exist for prevention and removal of intoxication consequences developed in living organisms exposed to OPs. The development of biologics for degradation of OPs and biotechnologies for their application in agriculture still represents an important task. New biologics based on stabilized forms of such enzyme as hexahistidine-tagged organophosphorus hydrolase (His6-OPH) have been in the form of nano-sized particles for OPs detoxification. They represent enzyme-polyelectrolyte complexes (EPC) obtained by mixing solutions of His6-OPH and polyanion under certain conditions. The main purpose of this work was to evaluate the usage efficiency of EPC based on His6-OPH and polyglutamic acid for OPs detoxification by analyzing biochemical blood parameters of rats fed with a grain mix containing chlorpyrifos. The experiment was conducted using female Sprague-Dowly albino rats. Treatment of the feeding grain mix initially containing chlopyrifos (48 mg/kg of the mix) with EPC based on His6-OPH (1000 U/kg of the mix) for 24 h was the most effective. The results showed that acetyl cholinesterase activity in blood of rats from the group consuming food after the enzymatic removal of chlorpyrifos, was comparable to acetyl cholinesterase activity in blood of rats consuming pure food.
Similar content being viewed by others
References
Aimaletdinov, A.M., Aslanov, R.M., Gareev, R.D., and Malan’ev, A.V., Veterenarnaya Meditsina, 2013, vol. 97, pp. 412–413.
Gómez-Pérez, M.L., Romero-González, R., Martínez Vidal, J.L., and Garrido Frenich, A., Food Addit. Contam., 2015, part A, pp. 1–10, doi 10.1080/19440049.2015.1023742
Mesnage, R., Defarge, N., Rocque, L.M., Spiroux de Vendômois, J., and Séralini, G.E., PLoS One, 2015, vol. 10, no. 7. e0128429. doi 10.1371/journal. pone.0128429
Eddleston, M., Buckley, N.A., Eyer, P., and Dawson, A.H., Lancet, 2008, vol. 371, no. 9612, pp. 597–607. doi 10.1016/S0140-6736(07)61202-1
Margni, M., Rossier, D., Crettaz, P., and Jolliet, O., Agric. Ecosyst. Environ., 2002, vol. 93, no. 1, pp. 379–392. doi 10.1016/S0167-8809(01)00336-X
Bhanti, M. and Taneja, A., Chemosphere, 2007, vol. 69, no. 1, pp. 63–68. doi 10.1016/j.chemosphere. 2007.04.071
Nigg, H.N. and Knaak, J.B., Reviews of Environmental Contamination and Toxicology, Whitacre, D.M., Ed., New York: Springer, 2005, pp. 29–111.
Ballantyne, B. and Marrs, T.C., Clinical and Experimental Toxicology of Organophosphates and Carbamates, Oxford: Butterworth Heinemann, 2017.
Efremenko, E.N., Votchitseva, Yu.A., Aliev, T.K., and Varfolomeev, S.D., Rus. Patent no. 2255975, Byul. Izobret., 2005, no. 19.
Efremenko, E., Votchitseva, Y., Plieva, F., Galaev, I., and Mattiasson, B., Appl. Microbiol. Biot., 2006, vol. 70, no. 5, pp. 558–563. doi 10.1007/s00253-005-0103-x
Maslova, O., Aslanli, A., Stepanov, N., Lyagin, I., and Efremenko, E., Catalysts, 2017, vol. 7, pp. 271–284. doi 10.3390/catal7090271
Lyagin, I.V., Efremenko, E.N., and Kabanov, A.V., Moscow Univ. Chem. Bull., 2014, vol. 55, no. 3, pp. 167–173. doi 10.3103/S0027131414030055
Lyagin, I.V., Andrianova, M.S., and Efremenko, E.N., Applied Microbiol. Biotechnol., 2016, vol. 100, no. 13, pp. 5829–5838. doi 10.1007/s00253-016-7407-x
Senko, O.V., Maslova, O.V., and Efremenko, E.N., Int. J. Environ. Res. Public Health, 2017, vol. 14, p. 1438. doi 10.3390/ijerph14121438
Efremenko, E.N., Lyagin, I.V., Klyachko, N.L., Bronich, T., Zavyalova, N.V., Jiang, Y., and Kabanov, A.V., J. Control. Release, 2017, vol. 247, pp. 175–181. doi 10.1016/j.jconrel.2016.12.037
Kabanov, A.V., Klyachko, N.L., Efremenko, E.N., and Lyagin, I.V., Rus. Patent no. 2575627, Byul. Izobret., 2014, no. 9.
Kabanov, A.V., Efremenko, E.N., Klyachko, N.L., Bronich, T.K., Lyagin, I.V., and Varfolomeev, S.D., Rus. Patent no. 2525658, Byul. Izobret., 2016, no. 5.
Ellman, G.L., Courtney, K.D., Andres, V., and Featherstone, R.M., Biochem. Pharmacol., 1961, vol. 7, no. 2, pp. 88–95.
Efremenko, E., Lyagin, I., Gudkov, D., and Varfolomeyev, S. Biocatal. Biotransformation, 2007, vol. 25, nos. 2–4, pp. 359–364. doi 10.1080/10242420701444389
Starostina, V.K. and Degteva, S.A., Kholinesterase: metody analiza i diagnosticheskoe znachenie (Cholinesterase: Methods of Analysis and Diagnostic Value), Vector-Best, Novosibirsk: Vector-best, 2008.
Singh, B.K. and Walker, A., FEMS Microbiol Rev., 2006, vol. 30, pp. 428–471. doi 10.1111/j.1574-6976. 2006.00018.x
Armbrust, K.L., Pest Manag Sci., 2001, vol. 57, no. 9, pp. 797–802. doi 10.1002/ps.361
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.V. Maslova, O.V. Senko, E.N. Efremenko, 2018, published in Biomeditsinskaya Khimiya.
Rights and permissions
About this article
Cite this article
Maslova, O.V., Senko, O.V. & Efremenko, E.N. The Influence of Enzymatic Removal of Chlorpyrifos from Feed Grain Mixes on Biochemical Parameters of Rat Blood. Biochem. Moscow Suppl. Ser. B 12, 181–185 (2018). https://doi.org/10.1134/S1990750818020087
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1990750818020087