Skip to main content
SpringerLink
Log in

Hepatic differentiation of adult and fetal liver stromal cells in vitro

  • Published:
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

The liver has a marked capacity for regeneration. In most cases the liver regeneration is determined by hepatocytes. The regenerative capacity of hepatocytes is significantly reduced in acute or chronic damage. For example, in patients with alcoholic cirrhosis repair mechanisms are not activated and only organ transplantation or advanced methods of regenerative medicine can help such patients. Clinical trials including patients with various forms of liver disease have shown promising results of transplantation of autologous bone marrow stem cells. However, improvement of the effectiveness of such treatment requires optimization of sources of progenitor cells. In this study we have isolated stromal cells from the liver biopsies of three patients with alcoholic cirrhosis, performed their morphological and phenotypic analysis, and evaluated the hepatic potential of these cells in vitro. Stromal cells isolated from the fetal liver were used for comparative evaluation. During hepatic differentiation both types of cells expressed hepatic markers and secreted albumin. These results can serve as a basis for the development of a new method for the treatment of end-stage liver disease. The stromal cells isolated from the liver biopsies proliferate for a long time in a culture and this provides opportunity to produce them in large amounts for subsequent differentiation into hepatocyte-like cells and autologous transplantation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Clotman, F. and Lemaigre, F.P., Cell Cycle, 2006, vol. 5, pp. 168–171. doi 10.4161/cc.5.2.2341

    Article  CAS  Google Scholar 

  2. Hewitt, N.J., Lechón, M.J., Houston, J.B., Hallifax, D., Brown, H.S., Maurel, P., Kenna, J.G., Gustavsson, L., Lohmann, C., Skonberg, C., et al., Drug Metab. Rev., 2007, vol. 39, pp. 159–234. doi 10.1080/03602530601093489

    Article  CAS  Google Scholar 

  3. Fox, I.J. and Roy-Chowdhury, J., J. Hepatol., 2004, vol. 40, pp. 878–886. doi 10.1016/j.jhep.2004.04.009

    Article  CAS  Google Scholar 

  4. Soltys, K.A., Soto-Gutiérrez, A., Nagaya, M., Baskin, K.M., Deutsch, M., Ito, R., Shneider, B.L., Squires, R., Vockley, J., Guha, C., et al., J. Hepatol., 2010, vol. 53, pp. 769–774. doi 10.1016/j.jhep.2010.05.010

    Article  Google Scholar 

  5. Bhogal, R.H., Hodson, J., Bartlett, D.C., Weston, C.J., Curbishley, S.M., Haughton, E., Williams, K.T., Reynolds, G.M., Newsome, P.N., Adams, D.H., et al., PLoS One, 2011, vol. 6, e18222. doi 10.1371/journal.pone.0018222

  6. Terry, C. and Hughes, R.D., Methods Mol. Biol., 2009, vol. 481, pp. 25–34. doi 10.1007/978-1-59745-201-4_3

    Article  CAS  Google Scholar 

  7. Gilchrist, E.S. and Plevris, J.N., Liver Transpl., 2010, vol. 16, pp. 118–129. doi 10.1002/lt.21965

    Article  Google Scholar 

  8. Yarygin, K., Lupatov, A., and Kholodenko, I., Clin. Interv. Aging., 2015, vol. 1, pp. 1909–1924. doi 10.2147/CIA.S97926

    Article  Google Scholar 

  9. Chen, Q., Khoury, M., Limmon, G., Choolani, M., Chan, J.K., and Chen, J., Stem Cells, 2013, vol. 31, pp. 1160–1169. doi 10.1002/stem.1359

    Article  CAS  Google Scholar 

  10. Jochheim-Richter, A., Rüdrich, U., Koczan, D., Hillemann, T., Tewes, S., Petry, M., Kispert, A., Sharma, A.D., Attaran, F., Manns, M.P., et al., Differentiation, 2006, vol. 74, pp. 167–173. doi 10.1111/j.1432-0436.2006.00066.x

    Article  CAS  Google Scholar 

  11. Moreno, R., Martinez-Gonzalez, I., Rosal, M., Farwati, A., Gratacos, E., and Aran, J.M., Stem Cells Dev., 2010, vol. 19, pp. 1579–1588. doi 10.1089/scd.2009.0514

    Article  CAS  Google Scholar 

  12. Haridass, D., Narain, N., and Ott, M., Curr. Opin. Organ Transplant., 2008, vol. 13, pp. 627–632. doi 10.1097/MOT.0b013e328317a44f

    Article  Google Scholar 

  13. Wang, J., Clark, J.B., Rhee, G.S., Fair, J.H., Reid, L.M., and Gerber, D.A., Cells Tissues Organs, 2003, vol. 173, pp. 193–203. doi 10.1159/000070375

    Article  Google Scholar 

  14. Oh, K., Shon, S.Y., Seo, M.W., Lee, H.M., Oh, J.-E., Choi, E.Y., Lee, D.-S., and Park, K.S., Exp. Mol. Med., 2015, vol. 47, e187. doi 10.1038/emm.2015.64

  15. Kuroda, Y., Kitada, M., Wakao, S., Nishikawa, K., Tanimura, Y., Makinoshima, H., Goda, M., Akashi, H., Inutsuka, A., Niwa, A., et al., Proc. Natl. Acad. Sci. USA, 2010, vol. 107, pp. 8639–8643. doi 10.1073/pnas.0911647107

    Article  CAS  Google Scholar 

  16. Kholodenko, I.V., Kholodenko, R.V., Manukyan, G.V., Burunova, V.V., and Yarygin, K.N., Klet. Tekhnol. Biol. Med., 2016, no. 3, pp. 147–151.

    Google Scholar 

  17. Shi, X., Chang, C.C., Basson, M.D., Uyhaa, A.L., Wei, L., and Zhang, P., J. Stem Cell Res. Ther., 2014, vol. 4, p. 205. doi 10.4172/2157-7633.1000205

  18. Forbes, S.J. and Parola, M., Best Pract. Res. Clin. Gastroenterol., 2011, vol. 25, pp. 207–217. doi 10.1016/j.bpg.2011.02.006

    Article  CAS  Google Scholar 

  19. Schmelzer, E., Liver Regeneration, Baptista P., Ed., InTech, 2012. ISBN: 978-953-51-0622-7.

  20. Lupatov, A.Yu., Vdovin, A.S., Vakhrushev, I.V., Poltavtseva, R.A., and Yarygin, K.N., Klet. Tekhnol. Biol. Med., 2014, no. 4, pp. 221–228.

    Google Scholar 

  21. Schmelzer, E., Wauthier, E., and Reid, L.M., Stem Cells, 2006, vol. 24, pp. 1852–1858. doi 10.1634/stemcells. 2006-0036

    Article  CAS  Google Scholar 

  22. Tanaka, M., Okabe, M., Suzuki, K., Kamiya, Y., Tsukahara, Y., Saito, S., and Miyajima, A., Mech. Dev., 2009, vol. 126, pp. 665–676. doi 10.1016/j.mod.2009.06.939

    Article  CAS  Google Scholar 

  23. Dollé, L., Theise, N.D., Schmelzer, E., Boulter, L., Gires, O., and van Grunsven, L.A., Am. J. Physiol. Gastrointest. Liver Physiol., 2015, vol. 308, pp. 233–250. doi 10.1152/ajpgi.00069.2014

    Article  Google Scholar 

  24. Sancho-Bru, P., Altamirano, J., Rodrigo-Torres, D., Coll, M., Millan, C., Lozano, J.J., Miquel, R., Arroyo, V., Caballeria, J., Gines, P., et al., Hepatology, 2012, vol. 55, pp. 1931–1941. doi 10.1002/hep.25614

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biomedical Chemistry (IBMC), ul. Pogodinskaya 10/8, Moscow, 119121, Russia

    I. V. Kholodenko & K. N. Yarygin

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences (IBCh RAS), Moscow, Russia

    R. V. Kholodenko

  3. Russian National Research Center of Surgery (RNRCS), Moscow, Russia

    G. V. Manukyan

Authors
  1. I. V. Kholodenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. V. Kholodenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. V. Manukyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. N. Yarygin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Kholodenko.

Additional information

Original Russian Text © I.V. Kholodenko, R.V. Kholodenko, G.V. Manukyan, K.N. Yarygin, 2017, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kholodenko, I.V., Kholodenko, R.V., Manukyan, G.V. et al. Hepatic differentiation of adult and fetal liver stromal cells in vitro. Biochem. Moscow Suppl. Ser. B 11, 234–242 (2017). https://doi.org/10.1134/S1990750817030052

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990750817030052

Keywords

Search

Navigation