Skip to main content
SpringerLink
Log in

The methylation status of GSTP1, APC, and RASSF1 genes in human prostate cancer samples: Comparative analysis of diagnostic informativeness of MS-HRM and hybridization on the Illumina Infinium HumanMethylation450 BeadChip

  • Published:
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

There is a clear need in molecular markers of prostate cancer (PC) that would specify stratification of patients into risk categories and serve as additional parameters to clinical prognostic factors. Altered DNA methylation status is one of molecular processes that occur during PC development. The gene methylation status may be evaluated in laboratory diagnostics by a PCR approach known as means of methylation-sensitive high resolution melting (MS-HRM) curve analysis. This method is particularly promising due to minimal amounts of DNA required for analysis (10 ng). To date, numerous data based on the use of Infinium HumanMethylation450 BeadChip (HM450) technology have been accumulated on DNA methylation in PC samples. However, it remains unclear whether the MS-HRM results are consistent with chip hybridization data. The aim of this study was to perform the comparative analysis of the diagnostic informativeness of MS-HRM and HM450 chip hybridization methods in determination of the methylation status of three genes GSTP1, APC, and RASSF1 in samples obtained from PC patients. The study has shown that using the MS-HRM method it is possible to discriminate PC tumor samples from samples of histologically unaltered prostate tissue by analyzing the methylation status of each gene. Analysis of chip hybridization data confirmed the results obtained by MS-HRM. Differences between tumor tissue and histologically intact tissue obtained from each PC patient in the methylation levels of GSTP1, APC, and RASSF1, determined by MS-HRM or chip hybridization were consistent with each other. Thus, the assessment of the methylation level of gene GSTP1, APC, and RASSF1 using MS-HRM can become the basis for the development of a laboratory test, the specifying diagnosis of prostate cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ferlay, J., Soerjomataram, I., Dikshit, R., Eser, S., Mathers, C., Rebelo, M., Parkin, D.M., Forman, D., and Bray, F., Int. J. Cancer, 2015, vol. 136, no. 5, pp. E359–E386. doi 10.1002/ijc.29210

    Article  CAS  Google Scholar 

  2. Kaprin, A.D., Starinskii, V.V., and Petrova, G.V., Sostoyanie onkologicheskoi pomoshchi naseleniyu Rossii v 2015 gody (The State of Oncological Care for the Population of Russian Federation in 2015), Moscow: Hertsen Moscow Oncology Research Institute, 2016.

    Google Scholar 

  3. Humphrey, P.A., Moch, H., Cubilla, A.L., Ulbright, T.M., and Reuter, V.E., Eur. Urol., 2016, vol. 70, no. 1, pp. 106–119. doi 10.1016/j.eururo.2016.02.028

    Article  Google Scholar 

  4. Van Neste, L., Partin, A. W., Stewart, G.D., Epstein, J.I., Harrison, D.J., and Van Criekinge, W., Prostate, 2016, vol. 76, no. 12, pp. 1078–1087. doi 10.1002/pros.23191

    Article  Google Scholar 

  5. Hanahan, D. and Weinberg, R.A., Cell, 2011, vol. 144, no. 5, pp. 646–674. http://dx.doi.org/doi 10.1016/j.cell.2011.02.013

    Article  CAS  Google Scholar 

  6. Berdasco, M. and Esteller, M., Dev. Cell, 2010, vol. 19, no. 5, pp. 698–711. doi 10.1016/j.devcel.2010.10.005

    Article  CAS  Google Scholar 

  7. Rybicki, B.A., Rundle, A., Kryvenko, O.N., Mitrache, N., Do, K.C., Jankowski, M., Chitale, D.A., Trudeau, S., Belinsky, S.A., and Tang, D., Int. J. Cancer, 2016, vol. 138, no. 12, pp. 2884–2893. doi 10.1002/ijc.30038

    Article  CAS  Google Scholar 

  8. Yang, B., Bhusari, S., Kueck, J., Weeratunga, P., Wagner, J., Leverson, G., Huang, W., and Jarrard, D.F., Neoplasia, 2013, vol. 15, vol. 4, pp. 399–408. doi 10.1593/neo.13280

    Article  CAS  Google Scholar 

  9. Kobayashi, Y., Absher, D.M., Gulzar, Z.G., Young, S.R., McKenney, J.K., Peehl, D.M., Brooks, J.D., Myers, R.M., and Sherlock, G., Genome Res., 2011, vol. 21, no. 7, pp. 1017–1027. doi 10.1101/gr.119487.110

    Article  CAS  Google Scholar 

  10. Mahapatra, S., Klee, E.W., Young, C.Y., Sun, Z., Jimenez, R.E., Klee, G.G., Tindall, D.J., and Donkena, K.V., Clin. Cancer Res., 2012, vol. 18, no. 10, pp. 2882–2895. doi 10.1158/1078–0432.CCR-11-2090

    Article  CAS  Google Scholar 

  11. Kim, J.W., Kim, S.T., Turner, A.R., Young, T., Smith, S., Liu, W., Lindberg, J., Egevad, L., Gronberg, H., Isaacs, W.B., and Xu, J., PLoS One, 2012, vol. 7, no. 10, e48455. doi 10.1371/journal. pone.0048455

    Google Scholar 

  12. Haldrup, C., Mundbjerg, K., Vestergaard, E.M., Lamy, P., Wild, P., Schulz, W.A., Arsov, C., Visakorpi, T., Borre, M., Høyer, S., Ørntoft, T.F., and Sørensen, K.D., J. Clin. Oncol., 2013, vol. 31, no. 26, pp. 3250–3258. doi 10.1200/JCO.2012.47.1847

    Article  CAS  Google Scholar 

  13. Geybels, M.S., Zhao, S., Wong, C.J., Bibikova, M., Klotzle, B., Wu, M., Ostrander, E.A., Fan, J.B., Feng, Z., and Stanford, J.L., Prostate, 2015, vol. 75, no. 16, pp. 1941–1950. doi 10.1002/pros.23093

    Article  CAS  Google Scholar 

  14. Hessels, D. and Schalken, J.A., Asian J. Androl., 2013, vol. 15, no. 3, pp. 333–339. doi 10.1038/aja.2013.6

    Article  CAS  Google Scholar 

  15. Henrique, R., Ribeiro, F.R., Fonseca, D., Hoque, M.O., Carvalho, A.L., Costa, V.L., Pinto, M., Oliveira, J., Teixeira, M.R., Sidransky, D., and Jerónimo, C., Clin. Cancer Res., 2007, vol. 13, no. 20, pp. 6122–6129. doi 10.1158/1078-0432.CCR-07-1042

    Article  CAS  Google Scholar 

  16. Litovkin, K., Van Eynde, A., Joniau, S., Lerut, E., Laenen, A., Gevaert, T., Spahn, M., Kneitz, B., Gramme, P., Helleputte, T., Isebaert, S., Haustermans, K., and Helleputte, T., PLoS One, 2015, vol. 10, no. 6, e0130651. doi 10.1371/journal.pone.0130651

    Article  Google Scholar 

  17. Babalyan, K.A., Sultanov, R., Generozov, E.V., Zakharzhevskaya, N.B., Sharova, E.I., Peshkov, M.N., Vasil’ev, A.O., Govorov, A.V., Pushkar’, D.Yu., Prilepskaya, E.A., Danilenko, S.A., Babikova, E.A., Larin, A.K., and Govorun, V.M., Vopr. Onkol., 2016, no. 1, pp. 122–132.

    Google Scholar 

  18. Wojdacz, T.K., Dobrovic, A., and Hansen, L.L., Nat. Protoc., 2008, vol. 3, no. 12, pp. 1903–1908. doi 10.1038/nprot.2008.191

    Article  CAS  Google Scholar 

  19. Spitzwieser, M., Holzweber, E., Pfeiler, G., Hacker, S., and Cichna-Markl, M., Breast Cancer Res., 2015, vol. 17, p. 125. doi 10.1186/s13058-015-0637-5

    Article  Google Scholar 

  20. Migheli, F., Stoccoro, A., Coppede, F., Omar, W.A.W., Failli, A., Consolini, R., Seccia, M., Spisni, R., Miccoli, P., Mathers, J.C., and Migliore, L., PLoS One, 2013, vol. 8, no. 1, e52501. doi 10.1371/journal. pone.0052501

    Article  CAS  Google Scholar 

  21. Assenov, Y., Müller, F., Lutsik, P., Walter, J., Lengauer, T., and Bock, C., Nat. Methods, 2014, vol. 11, no. 11, pp. 1138–1140. doi 10.1038/nmeth.3115

    Article  CAS  Google Scholar 

  22. Teschendorff, A.E., Marabita, F., Lechner, M., Bartlett, T., Tegner, J., Gomez-Cabrero, D., and Beck, S., Bioinformatics, 2013, vol. 29, no. 2, pp. 189–196. doi 10.1093/bioinformatics/bts680

    Article  CAS  Google Scholar 

  23. Triche, T.J., Weisenberger, D.J., Van Den Berg, D., Laird, P.W., and Siegmund, K.D., Nucleic Acids Res., 2013, vol. 41, no. 7, e90. doi 10.1093/nar/gkt090

    Article  CAS  Google Scholar 

  24. Bilic-Zulle, L., Biochem. Med. (Zagreb), 2011, vol. 21, no. 1, pp. 49–52. PMID: 22141206

    Article  Google Scholar 

  25. Giavarina, D., Biochem. Med. (Zagreb), 2015, vol. 25, no. 2, pp. 141–151. doi 10.11613/BM.2015.015

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Federal Research and Clinical Center of Physical-Chemical Medicine, ul. Malaya Pirogovskaya 1a, Moscow, 119435, Russia

    L. O. Skorodumova, S. A. Danilenko, E. V. Generozov, A. K. Larin, E. S. Kostryukova & E. I. Sharova

  2. Moscow Institute of Physics and Technology (State University), Dolgoprudniy, Moscow region, Russia

    K. A. Babalyan, R. Sultanov & E. S. Kostryukova

  3. Evdokimov Moscow State Medical Stomatological University (MSMSU), Moscow, Russia

    A. O. Vasiliev, A. V. Govorov, D. Y. Pushkar & E. A. Prilepskaya

Authors
  1. L. O. Skorodumova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. A. Babalyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Sultanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. O. Vasiliev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. V. Govorov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. Y. Pushkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. A. Prilepskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. S. A. Danilenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. E. V. Generozov
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. A. K. Larin
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. E. S. Kostryukova
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. E. I. Sharova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. O. Skorodumova.

Additional information

Original Russian Text © L.O. Skorodumova, K.A. Babalyan, R. Sultanov, A.O. Vasiliev, A.V. Govorov, D.Y. Pushkar, E.A. Prilepskaya, S.A. Danilenko, E.V. Generozov, A.K. Larin, E.S. Kostryukova, E.I. Sharova, 2017, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Skorodumova, L.O., Babalyan, K.A., Sultanov, R. et al. The methylation status of GSTP1, APC, and RASSF1 genes in human prostate cancer samples: Comparative analysis of diagnostic informativeness of MS-HRM and hybridization on the Illumina Infinium HumanMethylation450 BeadChip. Biochem. Moscow Suppl. Ser. B 11, 194–201 (2017). https://doi.org/10.1134/S199075081702007X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S199075081702007X

Keywords

Search

Navigation