Activity of liver mitochondrial Krebs cycle NAD+-dependent dehydrogenases in rats with hepatitis induced by acetaminophen under conditions of alimentary protein deficiency
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Activity of isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, malate dehydrogenase, and the NAD+/NADН ratio were studied in the liver mitochondrial fraction of rats with toxic hepatitis induced by acetaminophen under conditions of alimentary protein deficiency. Acetaminophen-induced hepatitis was characterized by a decrease of isocitrate dehydrogenase, α-ketoglutarate dehydrogenase and malate dehydrogenase activities, while the mitochondrial NAD+/NADН ratio remained at the control level. Modeling of acetaminophen-induced hepatitis in rats with alimentary protein deficiency caused a more pronounced decrease in the activity of studied Krebs cycle NAD+-dependent dehydrogenases and a 2.2-fold increase of the mitochondrial NAD+/NADН ratio.
Keywordsalimentary protein deficiency acetaminophen-induced hepatitis isocitrate dehydrogenase α-ketoglutarate dehydrogenase malate dehydrogenase NAD+/NADН ratio
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- 4.Semyachkina, A.N., Nikolaeva, E.A., Novikov, P.V., et al., Med. Genetika, 2002, vol. 4, pp. 186–190.Google Scholar
- 9.Kharchenko, N.V., Anokhina, G.A., and Kravchenko, V.V., Souchasna Gastroenterologiya, 2012, vol. 66, pp. 76–79.Google Scholar
- 15.Voloshchuk, O.N., Kopylchuk, G.P., and Kadaiskaya, T.G., Vopr. Pitaniya, 2014, vol. 83, pp. 12–16.Google Scholar
- 16.Rose, C.I. and Henderson, A.R., Clin. Chem., 1975, vol. 21, pp. 1619–1624.Google Scholar
- 22.Metody biokhimicheskikh issledovanyi (lipidnyi i energeticheskii obmen) (Methods of Biochemical Studies. Lipid and Energy Metabolism), ProkhorovaM.I.,ed., Leningrad: Leningrad University Press, 1982.Google Scholar
- 23.Singh, A., Bhat, T.K., and Sharma, O.P., J. Clinic. Toxicol., 2011, S4:001. doi 10.4172/2161-0495.S4-001Google Scholar