Abstract
Methemoglobin formation was examined in erythrocytes of 16 patients with Parkinson’s disease (PD) (stage 3–4 by the Hoehn and Yahr scale). The patients receiving levodopa-containing drugs (madopar, nakom) were also treated with intramuscular injections of mexidol (daily dose 100 mg/day) for 14 days. Control group included 12 clinically healthy persons. The erythrocyte methemoglobin content was determined by electronic paramagnetic resonance (EPR) using the EPR signal intensity with the g-factor 6.0. The methemoglobin content was significantly higher in erythrocytes of PD patients than in healthy donors. The complex therapy with mexidol normalized the methemoglobin content in erythrocytes of PD patients. Incubation in vitro of erythrocytes of donors and PD patients with acrolein increased the methemoglobin content, while incubation with carnosine normalized the methemoglobin content in erythrocytes of PD patients. Prophylactic (i.e. before acrolein addition) and therapeutic administration of carnosine to the incubation system with acrolein decreased the methemoglobin content to its initial level. Results of this study suggest that inclusion of the antioxidants mexidol and carnosine in the scheme of basic therapy of PD may reduce side effects associated with methemoglobinemia.
Similar content being viewed by others
References
Hwang, O., Exp. Neurobiol., 2013, vol. 22, pp. 11–17.
Danielson, S.R. and Andersen, J.K., Free Radic. Biol. Med., 2008, vol. 44, pp. 1787–1794.
Jomova, K., Vondrakova, D., Lawson, M., and Valko, M., Mol. Cell Biochem., 2010, vol. 345, pp. 91–104.
Illarioshkin, S.N., Zagorovskaya, I.A., Ivanova-Smolenskaya, I.A., and Markova, E.D., Nevrol. Zhurn., 2002, no. 5, pp. 47–51.
Zagorovskaya, T.B., Ivanova-Smolenskaya, I.A., Markova, E.D., Illarioshkin, S.N., and Bris, A., Nevrol. Zhurn., 2001, no. 4, pp. 13–18.
Illarioshkin, S.N., Shadrina, M.I., Bagyeva, G.Kh., Zagorovskaya, T.B., Markova, E.D., Karabanov, A.V., Poleshchuk, V.V., Polevaya, E.V., Fedorova, N.V., Limborskaya, S.A., and Ivanova-Smolenskaya, I.A., Annaly Kliniocheskoi i Eksperimental’noi Nevrologii, 2007, vol. 1, pp. 23–31.
Reutov, V.P., Sorokina, E.G., Okhotin, V.E., and Kositsyn, N.S., Tsiklicheskie prevrashcheniya oksida azota v organizme mlekopirayushchikh (Cyclic Conversions of Nitric Oxide in Mammalian Organisms), Moscow: Nauka, 1998.
Mohorovic, L., Lavezzi, A.M., Stifter, S., Perry, G., Malatestinic, D., Micovic, V., Materijan, E., Haller, H., and Petrovic, O., Advances in Bioscience and Biotechnology., 2014, vol. 5, no. 1. doi 10.4236/abb.2014.51003
Hare, G.M.T., Tsui, A.K.Y., Crawford, J. H., and Patel, R.P., Redox Biology, 2014, vol. 1, no. 10, pp. 65–69.
Ferrali, M., Signorini, C., Caciotti, B., Sugherini, L., Ciccoli, L., Giachetti, D., and Comporti, M., FEBS Letters, 1997, vol. 416, no. 2, pp. 123–129.
Dang, T.N., Arseneault, M., Murthy, V., and Ramassamy, C., Curr. Mol. Pharmacol., 2010, vol. 3, pp. 66–78.
Voronina, T.A., Korsakov Zhurn. Nevrol. Psikhiatr., 2012, no. 12, pp. 86–90.
Pulatova, M.K., Rikhireva, G.T., and Kuropteva, Z.V., Elektronnyi paramagnitnyi rezonanse v molekulyarnoi radiobiologii (Electronic Paramagnetic Resonance in Radiobiology), Moscow: Energoatomizdat, 1989.
Boldyrev, A.A., Biochemistry (Moscow), 2012, vol. 77, pp. 405–420.
Fedorova, T.N., Bagyeva, G.Kh., Dobrotvorskaya, I.S., Stepanova, M.S., Polevaya, E.V., Ivanova-Smolenskaya I.A., and Illarioshkin, S.N., Eksper. Klin. Farmakol., 2012, vol. 75, no. 6, pp. 23–26.
Katunina, E.A., Malykhina, N.V., Kuznetsov, G.N., Avakyan, E.I., Gusev, L.N., Voronina, T.A., and Barskov, I.V., Korsakov Zhurn. Nevrol. Psikhiatr., 2006, no. 9, pp. 22–28.
Shul’gin, A.V., Korsakov Zhurn. Nevrol. Psikhiatr., 2012, no. 2, pp. 35–39.
Licker, V., Cote, M., Lobrinus, J.A., Rodrigo, N., Kovari, E., Hochstrasser, D.F., Turck, N., Sanchez, J.C., and Burkhard, P.R., J. Proteomics, 2012, vol. 75, pp. 4656–4667.
Kang, J.H., Bull. Korean Chem. Soc., 2008, vol. 29, no. 9, pp. 1732–1736.
Konovalova, E.V., Fedorova, T.N., Makletsova, M.G., and Berezov, T.T., Vopr. Biol. Med. Farm. Khim., 2013, no. 6, pp. 43–48.
Carini, M., Aldini, G., Beretta, G., Arlandini, E., and Facino, R.M., J. Mass Spectrom., 2003, vol. 38, no. 9, pp. 996–1006.
Aldini, G., Facino, R.M., Beretta, G., and Carini, M., Biofactors, 2005, vol. 24, pp. 77–87.
Boldyrev, A., Fedorova, T., Stepanova, M., Dobrotvorskaya, I., Kozlova, E., Boldanova, N., Bagyeva, G., Ivanova-Smolenskaya, I., and Illarioshkin, S., Rejuvenation Res., 2008, vol. 11, pp. 821–827.
Tate, S.S., Orlando, J., and Meister, A., Proc. Natl. Acad. Sci. USA, 1972, vol. 69, p. 2505.
Mariani, S., Ventriglia, M., Simonelli, I., Spalletta, G., Bucossi, S., Siotto, M., Assogna F., Melgari, J.M., Vernieri, F., and Squitti, R., Front. Aging Neurosci., 2013, vol. 5, no. 37, pp. 1–7.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M.G. Makletsova, G.T. Rikhireva, V.V. Poleshuk, K.V. Gryakalov, S.L. Timerbaeva, T.N. Fedorova, 2016, published in Biomeditsinskaya Khimiya.
Rights and permissions
About this article
Cite this article
Makletsova, M.G., Rikhireva, G.T., Poleshuk, V.V. et al. The effect of antioxidants on in vivo and in vitro methemoglobin formation in erythrocytes of patients with Parkinson’s disease. Biochem. Moscow Suppl. Ser. B 10, 264–268 (2016). https://doi.org/10.1134/S1990750816030124
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1990750816030124