Abstract
The aim of this study was to determine reference values of matrix metalloproteinases (MMPs) MMP-1, MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in the amniotic fluid at the first stage of labor in physiological pregnancy. Eighty nine women at the first stage of term labor have been examined. Samples of amniotic fluid were taken at the first period of labor by vaginal amniotomy. Concentrations of MMP-1, MMP-2, MMP-9, and TIMP-1 have been investigated in amniotic fluid samples by ELISA kits. The following normal concentration ranges for MMP-1, MMP-2, MMP-9, TIMP-1, and ratios of concentrations of MMPs and TIMP-1 (MMP-1/TIMP-1, MMP-2/TIMP-1, MMP-9/TIMP-1) have been determined for amniotic fluid samples obtained during the first period of labor in physiological pregnancy. These included: MMP-1: 5.1–16.8 pg/mg of protein; MMP-2: 238.3–374.1 pg/mg of protein; MMP-9: 66.1–113.3 pg/mg of protein, TIMP-1: 4.7–13.6 pg/mg of protein, MMP-1/TIMP-1 ratio: 0.1–2.2, MMP-2/TIMP-1 ratio: 19.9–55.7; MMP-9/TIMP-1 ratio: 4.2–17.2.
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Torrance, H.L., Pistorius, L., Voorbij, H.A., and Visser, G.H., J. Matern. Fetal Neonatal Med., 2013, vol. 26, pp. 728–730. doi 10.3109/14767058. 2013.766685
Wiberg-Itzel, E., Akerud, H., Andolf, E., Hellstrom-Westas, L., and Winbladh, B., Wennerholm, U.-B., Obstet. Gynecol., 2011, vol. 118, pp. 135–142. doi 10.1097/AOG.0b013e318220c0d4
Weiss, A., Goldman, S., and Shalev, E., Frontiers in Bioscience, 2007, vol. 12, pp. 649–659. doi 10.2741/2089
Cockle, J.V., Gopichandran, N., Walker, J.J., Levene, M.I., and Orsi, N.M., Reprod. Sci., 2007, vol. 14, pp. 629–645. doi 10.1177/1933719107304563
Goldman, S., Weiss, A., Eyali, V., and Shalev, E., Mol. Hum. Reprod., 2003, vol. 9, pp. 367–373. doi 10.1093/molehr/gag040
Xu, P., Alfaidy, N., and Challis, J.R.G., J. Clin. Endocrinol. Metab., 2002, vol. 87, pp. 1353–1361. doi 10.1210/jcem.87.3.8320
Vadillo-Ortega, F. and Estrada-Gutiérrez, G., BJOG, 2005, vol. 112, Suppl. 1, pp. 19–22. doi 10.1111/j.1471-0528.2005.00579.x
Pupkova, V.I. and Prasolova, L.M., Opredelenie belka v moche i spinomozgovoi zhidkosti (Protein Determination in Urine and Cerebrospinal Fluid), Kol’tsovo, 2007.
Baker, A.H., Edwards, D.R., and Murphy, G., J. Cell Sci., 2002, vol. 115, pp. 3719–3727. doi 10.1242/jcs.00063
Hutton, M., Willenbrock, F., Brocklehurst, K., and Murphy, G., Biochemistry, 1998, vol. 37, no. 28, pp. 10094–10098. doi 10.1021/bi980616p
Korenovsky, Yu.V., Sinel’nikova, L.M., Fil’chakova, O.N., Shabalina, Yu.V., Ershova, E.G., Fadeeva, N.I., and El’chaninova, S.A., Byull. Vostochno-Sibirskogo Nauchnogo Tsentra, 2012, no. 5(87), Part 2, pp. 146–149.
Brace, R.A. and Cheung, C.Y., Adv. Exp. Med. Biol., 2014, vol. 814, pp. 49–68. doi 10.1007/978-1-4939-1031-1_5
Turner, S.W., Carter, J., Danielian, P., Chalmers, I., McConaghy, L., Pacitti, N., and Booth, N., J. Matern. Fetal Neonatal Med., 2014, vol. 27, pp. 416–420. doi 10.3109/14767058.2013.818647
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Original Russian Text © Yu.V. Korenovsky, O.V. Remneva, 2016, published in Biomeditsinskaya Khimiya.
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Korenovsky, Y.V., Remneva, O.V. Reference values of concentrations of matrix metalloproteinases-1, -2, -9 and the tissue inhibitor of matrix metalloproteinases (TIMP-1) in the amniotic fluid during physiological pregnancy and delivery. Biochem. Moscow Suppl. Ser. B 10, 240–242 (2016). https://doi.org/10.1134/S1990750816030070
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DOI: https://doi.org/10.1134/S1990750816030070