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Proteomic analysis of exhaled breath condensate for diagnostics of respiratory system diseases

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Abstract

Study of proteomic composition of exhaled breath condensate (EBC) is a promising non-invasive method for diagnostics of respiratory system diseases in patients. In this study the EBC proteomic composition of 53 donors, including patients with different respiratory system diseases has been investigated. Cytoskeletal keratins type II (1, 2, 3, 4, 5, 6) and cytoskeletal keratins type I (9, 10, 14, 15, 16) were invariant for all samples. Analyzing the frequency of occurrence of proteins in different groups of examined patients, several categories of proteins have been recognized: proteins found in all pathologies (dermcidin, alpha-1- microglobulin, SHROOM3), proteins simultaneously found in two groups (CSTA, LCN1, JUP, PIP, TXN), and proteins specific for a particular group (PRDX1, annexin A1/A2). The EBC analysis by HPLC-MS/MS can be used for identification of potential protein markers specific for inflammatory pulmonary diseases of infective origin (pneumonia) as well as for non-infectious diseases such as chronic obstructive pulmonary disease (COPD).

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Authors and Affiliations

  1. Research Center for Obstetrics, Gynecology and Perinatology, ul. Oparina 4, Moscow, 117997, Russia

    A. S. Kononikhin, N. L. Starodubtseva, I. A. Popov & M. G. Zavialova

  2. Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences (IBP RAS), ul. Kosygina 4, Moscow, 119934, Russia

    A. S. Kononikhin, K. Yu. Fedorchenko, A. M. Ryabokon, N. L. Starodubtseva, I. A. Popov, S. D. Varfolomeev & E. N. Nikolaev

  3. Lomonosov Moscow State University, Moscow, 119991, Russia

    K. Yu. Fedorchenko, A. M. Ryabokon & S. D. Varfolomeev

  4. Research Institute of Pulmonology, ul. 11-ya Parkovaya 32, Bld. 4, Moscow, 105077, Russia

    E. C. Anaev & A. G. Chuchalin

Authors
  1. A. S. Kononikhin
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  2. K. Yu. Fedorchenko
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  3. A. M. Ryabokon
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  4. N. L. Starodubtseva
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  5. I. A. Popov
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  6. M. G. Zavialova
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  7. E. C. Anaev
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  8. A. G. Chuchalin
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  9. S. D. Varfolomeev
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  10. E. N. Nikolaev
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Corresponding author

Correspondence to A. S. Kononikhin.

Additional information

Original Russian Text © A.S. Kononikhin, K.Yu. Fedorchenko, A.M. Ryabokon, N.L. Starodubtseva, I.A. Popov, M.G. Zavialova, E.C. Anaev, A.G. Chuchalin, S.D. Varfolomeev, E.N. Nikolaev, 2016, published in Biomeditsinskaya Khimiya.

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Kononikhin, A.S., Fedorchenko, K.Y., Ryabokon, A.M. et al. Proteomic analysis of exhaled breath condensate for diagnostics of respiratory system diseases. Biochem. Moscow Suppl. Ser. B 10, 230–234 (2016). https://doi.org/10.1134/S1990750816030069

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  • DOI: https://doi.org/10.1134/S1990750816030069

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