Skip to main content

Lipid peroxidation in cardiac mitochondrial fraction of rats exposed to different supplementation with polyunsaturated fatty acids

Abstract

The effect of diet supplementation with polyunsaturated fatty acids (PUFAs) used at different ratios of ω-6/ω-3 on the content of primary (diene conjugates, DC; triene conjugates, TC), secondary (ketodienes, CD; coupled trienes, CT; TBA-active products) and terminal (Schiff bases) lipid peroxidation products (LPO) and generation of superoxide anion-radical was studied in rat cardiac mitochondrial fraction. The cardiac mitochondrial fraction of rats kept on a diet with a high content of ω-6 and ω-3 PUFAs for eight weeks was characterized by increased content the primary, secondary and final LPO and a higher rate of superoxide radical formation. In the case of diet supplementation with ω-6 and ω-3 PUFAs used at the ratio of 4: 1, the leading factor determining LPO intensity in the cardiac mitochondrial fraction is a species PUFA composition rather than the degree of saturation.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    Tret’yak, N.N., Anoshina, M.Yu., Moshinskaya, O.V., et al., Onkologiya, 2002, vol. 4, pp. 29–32.

    Google Scholar 

  2. 2.

    Kambachakova, Z.A., Zhurn. Infektol., 2011, vol. 3, pp. 63–67.

    Google Scholar 

  3. 3.

    Bruins, M.J., Dane, A.D., Strassburg, K., et al., J. Lipid. Res., 2013, vol. 54, pp. 1598–1607.

    CAS  Article  Google Scholar 

  4. 4.

    Lawson, J.A., Kim, S., Powell, W.S., FitzGerald, G.A., and Rokach, J., J. Lipid Res., 2006, vol. 47, pp. 2515–2524.

    CAS  Article  Google Scholar 

  5. 5.

    Roberts, L.J. and Milne, G.L., J. Lipid Res., 2009, vol. 50, pp. 219–223.

    Article  Google Scholar 

  6. 6.

    Simopoulos, A.P., Exp. Biol. Med. (Maywood), 2008, vol. 233, pp. 674–688.

    CAS  Article  Google Scholar 

  7. 7.

    Simopoulos, A.P., Biomed. Pharmacother., 2006, vol. 60, pp. 502–507.

    CAS  Article  Google Scholar 

  8. 8.

    Wertz, P.W., Toxicol. and Health, 2009, vol. 25, pp. 279–283.

    CAS  Article  Google Scholar 

  9. 9.

    Reeves, P.G., Nielsen, F.H., and Fahey, G.C., J. Nutr., 1993, vol. 123, pp. 1939–1951.

    CAS  Google Scholar 

  10. 10.

    Kruatian, T. and Jitmanee, K., Chiang Mai J. Sci., 2013, vol. 40, pp. 419–426.

    CAS  Google Scholar 

  11. 11.

    Weinbach, T.C., Analyt. Biochem., 1961, vol. 2, pp. 335–343.

    CAS  Article  Google Scholar 

  12. 12.

    Azizova, G.I. and Efendiev, A.M., Biomed. Khim., 2009, vol. 55, pp. 779–783.

    CAS  Google Scholar 

  13. 13.

    Volchegorskii, I.A., Nalimov, I.A., Yarovinskii, B.G., and Lifshits, R.I., Vopr. Med. Khim., 1989, vol. 35, pp. 127–131.

    CAS  Google Scholar 

  14. 14.

    L’vovskaya, E.I., Volchegorskii, I.A., and Shemyakov, S.E., Vopr. Med. Khim., 1991, vol. 37, pp. 92–93.

    Google Scholar 

  15. 15.

    Gavrilov, V.B., Gavrilova, A.R., and Mazhul, L.M., Vopr. Med. Khim., 1987, vol. 33, pp. 118–122.

    CAS  Google Scholar 

  16. 16.

    Marchenko, M.M. and Ketsa, O.V., Ukr. Biokhim. Zhurn., 2012, vol. 84, pp. 95–102.

    Google Scholar 

  17. 17.

    Lowry, O.H., Rosebrough, M.J., Farr, A.L., and Randal, R.J., J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    CAS  Google Scholar 

  18. 18.

    Jump, D.B., Depner, C.M., and Tripathy, S., J. Lipid Res., 2012, vol. 53, pp. 2525–2545.

    CAS  Article  Google Scholar 

  19. 19.

    Tanito, M., Brush, R.S., Elliton, M.N., Wicker, L.D., Henry, K.R., and Anderson, R.E., J. Lipid Res., 2009, vol. 50, pp. 807–819.

    CAS  Article  Google Scholar 

  20. 20.

    Kandasamy, N., Joseph, F., and Goenka, N., Br. J. Diabet. Vasc. Dis., 2008, vol. 8, pp. 121–128.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to O. V. Ketsa.

Additional information

Original Russian Text © O.V. Ketsa, I.O. Shmarakov, M.M. Marchenko, 2016, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Ketsa, O.V., Shmarakov, I.O. & Marchenko, M.M. Lipid peroxidation in cardiac mitochondrial fraction of rats exposed to different supplementation with polyunsaturated fatty acids. Biochem. Moscow Suppl. Ser. B 10, 251–257 (2016). https://doi.org/10.1134/S1990750816030057

Download citation

Keywords

  • lipid peroxidation
  • superoxide anion-radical
  • polyunsaturated fatty acids
  • mitochondrial fraction
  • heart