Selection of DNA aptamers for breast cancer


A method of selection of DNA aptamers to breast tumor tissue based on the use of postoperative material has been developed. Breast cancer tissues were used as the positive target; the negative targets included benign tumor tissue, adjacent healthy tissues, breast tissues from mastopathy patients, and also tissues of other types of malignant tumors. During selection a pool of DNA aptamers demonstrating selective binding to breast cancer cells and tissues and insignificant binding to breast benign tissues has been obtained. These DNA aptamers can be used for identification of protein markers, breast cancer diagnostics, and targeted delivery of anticancer drugs.

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  1. 1.

    Pak, D.D., Usov, F.N., Fetisova, E.Yu., Volchenko, A.A., and Efanov, V.V., Onkologiya, 2013, vol. 4, pp. 34–39.

    Google Scholar 

  2. 2.

    Ferlay, J., Steliarova-Foucher, E., Lortet-Tieulent, J., Rosso, S., Coebergh, J.W.W., Comber, H., Forman, D., and Bray, F., Eur. J. Cancer, 2013, vol. 49, no. 6, pp. 1374–1403. doi

    CAS  Article  Google Scholar 

  3. 3.

    Kamangar, F., Dores, G.M., and Andersonm W.F., J. Clin. Oncol., 2006, vol. 24, pp. 2137–2150.

    Article  Google Scholar 

  4. 4.

    Watson, M.A., Dintzis, S., and Darrow, C.M., Cancer Res., 1999, vol. 59, pp. 3028–3031.

    CAS  Google Scholar 

  5. 5.

    Ross, J.S., Linette, G.P., Stec, J., Clark, E., Ayers, M., Leschly, N., Symmans, W.F., Hortobagyi, G.N., and Pusztai L., Expert Rev. Mol. Diagn., 2003, vol. 3, pp. 573–585.

    CAS  Article  Google Scholar 

  6. 6.

    Pultz, B.A., Luz, F.A.C., Faria, P.R., Oliveira, A.P.L., Araújo, R.A., and Silva, M.J.B., J. Cancer, 2014, vol. 5, pp. 559–571.

    CAS  Article  Google Scholar 

  7. 7.

    Misek, D.E. and Kim, E.H., Int. J. Proteomics, 2011, 343582, 9. doi

    Google Scholar 

  8. 8.

    Berezovski, M.V., Lechmann, M., Musheev, M.U., Mak, T.W., and Krylov, S.N., J. Am. Chem. Soc., 2008, vol. 130, pp. 9137–9143. doi 10.1021/ja801951p

    CAS  Article  Google Scholar 

  9. 9.

    Iliuk, A.B., Hu, L., and Tao, W.A., Anal. Chem., 2011, vol. 83, pp. 4440–4452.

    CAS  Article  Google Scholar 

  10. 10.

    Wehbe, M.B., Labib, M.A., Muharemagic, D.A., Zamay, A.S., and Berezovski, M.V., Biosens. Bioelectron., 2015, vol. 67, pp. 280–286.

    CAS  Article  Google Scholar 

  11. 11.

    Kolovskaya, O.S., Savitskaya, A.G., Zamay, T.N., Reshetneva, I.T., Zamay, G.S., Erkaev, E.N., Wang, X.B., Wehbe, M.B., Salmina, A.B., Perianova, O.V., Zubkova, O.A., Spivak, E.A., Mezko, V.S., Glazyrin, Yu.E., Titova, N.M., Berezovski, M.V., and Zamay, A.S., J. Medic. Chem., 2013, vol. 56, pp. 1564–1572.

    CAS  Article  Google Scholar 

  12. 12.

    Kolovskaya, O.S., Zamay, T.N., Zamay, A.S., Glazyrin, Yu. E., Spivak, E.A., Zubkova, O.A., Kadkina, A.V., Erkaev, E.N., Zamay, G.S., Savitskaya, A.G., Trufanova, L.V., Petrova, L.L., and Berezovsky, M.V., Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 2014, vol. 8, pp. 60–72.

    Article  Google Scholar 

  13. 13.

    Zamay, A.S., Zamay, G.S., Glazyrin, Yu.E., Zamay, T.N., Krat, A.V., Modestov, A.A., Zubkova, O.A., Spivak, E.A., Sukhovolskaia, M.A., Kuznetsova, S.A., Salmina, A.B., Gargaun, A.A., Berezovski, M.V., Kolovskaya, O.S., Oligos & Peptides-ChimicaOggi-Chemistry Today, 2014, vol. 32, pp. 24–28.

    CAS  Google Scholar 

  14. 14.

    Zamay, G.S., Kolovskaya, O.S., Zamay, T.N., Glazyrin, Y.E., Krat, A.V., Zubkova, O., Spivak, E., Wehbe, M., Gargaun, A., Muharemagic, D., Komarova, M., Grigorieva, V., Savchenko, A., Modestov, A.A., Berezovski, M.V., and Zamay, A.S., Molecular Therapy, 2015, vol. 23, pp. 1486–1496. doi 10.1038/mt.2015.108

    CAS  Article  Google Scholar 

  15. 15.

    Ruff, K.M., Snyder, T.M., and Liu, D.R., J. Amer. Chem. Soc., 2010, vol. 132, pp. 9453–9464.

    CAS  Article  Google Scholar 

  16. 16.

    Zhang, K., Tang, L., Sefah, K., Zhao, Z., Zhu, G., Sun, W., Goodison, S., and Tan, W., ChemMedChem, 2012, vol. 7, pp. 79–84.

    CAS  Article  Google Scholar 

  17. 17.

    Li, X., Zhang, W., Liu, L., Zhu, Z., Ouyang, G., An, Y., Zhao, C., and Yang, C.J., Anal. Chem., 2014, vol. 86, pp. 6596–6603. doi 10.1021/ac501205q

    CAS  Article  Google Scholar 

  18. 18.

    Hu, Y., Duan, J., Zhan, Q., Wang, F., Lu, X., and Yang, X.-D., PLoS One, 2012, vol. 7, e31970. doi 10.1371/journal.pone.0031970

    CAS  Article  Google Scholar 

  19. 19.

    Giangrande, P., McNamara, J., Thiel, K., Thiel, W., and Rockney, W., HER2 Nucleaic Acid Aptamers, 2013, US20130129719.

    Google Scholar 

  20. 20.

    Lee, Y.J., Kim, S., Park, S.-A., Kim, Y., Lee, J.E., Noh, D.-Y., Kim, K.-T., Ryu, S.H., and Suh, P.-G., Molecular Therapy, 2013, vol. 21, pp. 1004–1013. doi 10.1038/mt.2013.30

    CAS  Article  Google Scholar 

  21. 21.

    Shao, K., Ding, W., Wang, F., Li, H., Ma, D., and Wang, H., PLoS One, 2011, vol. 6, e24910.

    CAS  Article  Google Scholar 

  22. 22.

    Yufa, R., Krylova, S.M., Bruce, C., Bagg, E.A., Schofield, C.J., and Krylov, S.N., Anal. Chem., 2015, vol. 87, pp. 1411–1419.

    CAS  Article  Google Scholar 

  23. 23.

    Levay, A., Brenneman, R., Hoinka, J., Sant, D., Cardone, M., Trinchieri, G., Przytycka, T.M., and Berezhnoy, A., Nucl. Acids Res. 2015, vol. 43, no. 12, e82. doi 10.1093/nar/gkv534

    Article  Google Scholar 

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Correspondence to T. N. Zamay.

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Original Russian Text © G.S. Zamay, I.V. Belyanina, A.S. Zamay, M.A. Komarova, A.V. Krat, E.N. Eremina, R.A. Zukov, A.E. Sokolov, T.N. Zamay, 2016, published in Biomeditsinskaya Khimiya.

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Zamay, G.S., Belyanina, I.V., Zamay, A.S. et al. Selection of DNA aptamers for breast cancer. Biochem. Moscow Suppl. Ser. B 10, 158–164 (2016).

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  • DNA aptamers
  • oligonucleotides
  • breast cancer