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Analysis of proteomic profile changes of Danio rerio embryos during exposure to doxorubicin, incorporated in the phospholipid transport nanosystem

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Abstract

The proteome profile of Danio rerio embryos grown in the medium containing doxorubicin, included in the phospholipid transport nanosystem (doxolip) has been investigated using combination of 1D-electrophoresis with subsequent MALDI-TOF-PMF mass spectrometry. Cultivation of growing of D. rerio embryos in the medium with doxolip caused a substantial increase in expression of the cytoskeletal proteins, a decrease in the number of nuclear proteins involved in DNA and RNA synthesis and disappearance of vitellogenin 2 in comparison with control (the cultivation medium containing the phospholipid transport nanosystem). Analysis of the proteomic profiles of doxolip-treated embryos suggests lower toxicity of doxorubicin incorporated in the phospholipid nanosystem.

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Authors and Affiliations

  1. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia

    N. F. Samenkova, Yu. S. Kisrieva, N. A. Petushkova, G. P. Kuznetsova, O. V. Larina, O. P. Trifonova, I. I. Karuzina, O. M. Ipatova & A. V. Lisitsa

Authors
  1. N. F. Samenkova
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  2. Yu. S. Kisrieva
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  3. N. A. Petushkova
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  4. G. P. Kuznetsova
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  5. O. V. Larina
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  6. O. P. Trifonova
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  7. I. I. Karuzina
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  8. O. M. Ipatova
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  9. A. V. Lisitsa
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Corresponding author

Correspondence to Yu. S. Kisrieva.

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Original Russian Text © N.F. Samenkova, Yu.S. Kisrieva, N.A. Petushkova, G.P. Kuznetsova, O.V. Larina, O.P. Trifonova, I.I. Karuzina, O.M. Ipatova, A.V. Lisitsa, 2014, published in Biomeditsinskaya Khimiya.

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Samenkova, N.F., Kisrieva, Y.S., Petushkova, N.A. et al. Analysis of proteomic profile changes of Danio rerio embryos during exposure to doxorubicin, incorporated in the phospholipid transport nanosystem. Biochem. Moscow Suppl. Ser. B 8, 108–114 (2014). https://doi.org/10.1134/S1990750814020115

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  • DOI: https://doi.org/10.1134/S1990750814020115

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