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Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface

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Abstract

Applicability of scanning electron microscopy (SEM) for visualization of individual acts of DNA hybridization with oligonucleotide probes has been investigated using gold nanoparticles as a label. DNA or oligonucleotides were labeled with biotin molecules, which were then detected in DNA duplexes using a streptavidin conjugate with gold nanoparticles. Effective imaging of DNA duplexes was possible using the conjugate prepared by covalent binding. The detection limit of the model oligonucleotide of 19 bases was 20 pg.

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Authors and Affiliations

  1. Chemistry Faculty, Lomonosov Moscow State University, Leninskie Gory 1, bld.3, Moscow, 119991, Russia

    G. V. Presnova, M. Yu. Rubtsova, V. G. Grigorenko & A. M. Egorov

  2. Advanced Technologies Center, CSC, Moscow, 119311, Russia

    I. V. Yaminsky

  3. Immunotek, Moscow, Russia

    G. V. Presnova, M. Yu. Rubtsova, V. G. Grigorenko & A. M. Egorov

  4. Institute of Nuclear Physics, Lomonosov Moscow State University, Moscow, Russia

    D. E. Presnov

Authors
  1. G. V. Presnova
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  2. M. Yu. Rubtsova
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  3. D. E. Presnov
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  4. V. G. Grigorenko
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  5. I. V. Yaminsky
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  6. A. M. Egorov
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Corresponding author

Correspondence to M. Yu. Rubtsova.

Additional information

Original Russian Text © G.V. Presnova, M.Yu. Rubtsova, D.E. Presnov, V.G. Grigorenko, I.V. Yaminsky, A.M. Egorov, 2014, published in Biomeditsinskaya Khimiya.

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Presnova, G.V., Rubtsova, M.Y., Presnov, D.E. et al. Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface. Biochem. Moscow Suppl. Ser. B 8, 164–167 (2014). https://doi.org/10.1134/S1990750814020103

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  • DOI: https://doi.org/10.1134/S1990750814020103

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