Abstract
Metabolic activity of innate immune cells infected by various doses of Gram-negative (Yersinia pseudotuberculosis, Salmonella enteritidis) and Gram positive (Staphylococcus aureus, Listeria monocytogenes) bacteria has been investigated. Using various animal models we found that in during the initial period (up to 2 days) the changes in cellular responses depend on the type of the pathogen. In response to infection caused by Gram-negative bacteria predominant of neutrophil accumulation in the foci of inflammation was observed, while Gram-positive bacteria induced preferential accumulation of macrophages. The study of metabolism of these cells showed that the response of terminally differentiated primed phagocytes to pathogen appearance was higher than in cells circulating in blood. In addition to the priming state the phagocyte reactivity is influenced by the bacterial load. At a low phagocyte/microbe ratio the cells reaction is almost undetectable, while an excess of microorganisms causes (despite of the increase of the phagocytic parameters) the hyperactivation of cell metabolism and production of maximal amounts of bactericide agents, which exhibit a damaging effect on the cell itself.
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Original Russian Text © N.G. Plekhova, L.M. Somova, E.I. Drobot, 2014, published in Biomeditsinskaya Khimiya.
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Plekhova, N.G., Somova, L.M. & Drobot, E.I. Metabolism of innate immune cells in bacterial infections. Biochem. Moscow Suppl. Ser. B 8, 155–163 (2014). https://doi.org/10.1134/S1990750814020097
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DOI: https://doi.org/10.1134/S1990750814020097