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The SPR-based biosensor test system for analysis of small compounds interaction with human cytochrome P450 51A1 (CYP51A1)

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Abstract

The SPR-based test for human cytochrome P450 51A1 (CYP51A1) ligand screening has been developed. Applicability of this system has been validated with known azole inhibitors of cytochromes P450. The studied azoles selectively interacted with human cytochrome P450 51A1, which showed the highest affinity towards ketoconazole. The efficiency of the SPR based assay has been tested using 19 steroid and triterpene compounds, which have not been investigated as potential ligands of CYP51A1.

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Authors and Affiliations

  1. Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia

    O. V. Gnedenko, L. A. Kaluzhskiy, A. A. Molnar, A. S. Ivanov, A. V. Lisitsa & A. I. Archakov

  2. Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, Minsk, Belarus

    A. V. Yantsevich, D. V. Mukha, A. A. Gilep & S. A. Usanov

  3. Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia

    V. A. Stonik

Authors
  1. O. V. Gnedenko
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  2. L. A. Kaluzhskiy
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  3. A. A. Molnar
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  4. A. V. Yantsevich
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  5. D. V. Mukha
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  6. A. A. Gilep
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  7. S. A. Usanov
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  8. V. A. Stonik
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  9. A. S. Ivanov
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  10. A. V. Lisitsa
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  11. A. I. Archakov
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Correspondence to O. V. Gnedenko.

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Original Russian Text © O.V. Gnedenko, L.A. Kaluzhskiy, A.A. Molnar, A.V. Yantsevich, D.V. Mukha, A.A. Gilep, S.A. Usanov, V.A. Stonik, A.S. Ivanov, A.V. Lisitsa, A.I. Archakov, 2013, published in Biomeditsinskaya Khimiya.

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Gnedenko, O.V., Kaluzhskiy, L.A., Molnar, A.A. et al. The SPR-based biosensor test system for analysis of small compounds interaction with human cytochrome P450 51A1 (CYP51A1). Biochem. Moscow Suppl. Ser. B 7, 187–195 (2013). https://doi.org/10.1134/S1990750813030049

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  • DOI: https://doi.org/10.1134/S1990750813030049

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