Functional activity of the NADH-dependent reductase system in liver and Guerin’s carcinoma microsomal fraction in rats exposed to preliminary irradiation

  • M. M. Marchenko
  • O. V. KetsaEmail author


The activity of liver microsomal and Guerin’s carcinoma NADH-cytochrome b 5 reductase, the content and the rate of cytochrome b 5 oxidation-reduction have been investigated in tumor-bearing rats exposed to preliminary irradiation. Preliminary irradiation of rats (before transplantation of Guerin’s carcinoma) resulted in the decrease of NADH-cytochrome b 5 reductase activity and the content of cytochrome b 5 in the Guerin’s carcinoma microsomal fraction in the latent and logarithmic phases of oncogenesis compared with the non-irradiated tumor-bearing rats. The effect of irradiation preceding transplantation of the tumor to rats results in the increase of enzymatic activities of liver microsomal NADH-cytochrome b 5 reductase in the latent and logarithmic phases of tumor growth as compared with non-irradiated tumor-bearing rats. At the same time the content of cytochrome b 5 decreased, while the rate of its oxidation-reduction rate simultaneously increased in the liver microsomal fraction of tumor-bearing rats.


NADH-cytochrome b5 reductase cytochrome b5 microsomal fraction liver Guerin’s carcinoma 


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© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  1. 1.Fedkovich Chernovtsy National UniversityChernovtsyUkraine

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