Skip to main content

Arginine and lysine as products of basic carboxypeptidase activity associated with fibrinolysis

Abstract

Blood carboxypeptidases play an important role in the regulation of fibrinolysis. We have proposed here the method for the assay of blood carboxypeptidase activity associated with coagulation/fibrinolysis using the natural substrate fibrin and the detection of basic amino acids arginine and lysine as products under conditions closely resembling those in vivo. Plasma samples from 15 patients with arterial hypertension have been investigated. Coagulation and subsequent fibrinolysis were initiated by addition of standard doses of thrombin and tissue plasminogen activator, respectively. Arginine and lysine concentrations before, during, and after completion of fibrinolysis were determined using HPLC. The parameters of fibrinolysis were evaluated by the clot turbidity assay. The coagulation/fibrinolysis cycle was accompanied by a significant increase in concentrations of arginine and lysine in the incubation mixture by 101 and 81%, respectively. The duration of fibrinolysis initiation significantly correlated with the degree of increase of these amino acids: r S = −0.733 and −0.761 for arginine and lysine, respectively (p < 0.05). Arginine generation had two maximums: one in the beginning of clot lysis and another one at the end of the lysis, whereas the lysine release occurred mainly in the middle of fibrinolysis. Thus, the carboxypeptidase activity associated with fibrinolysis can be considered as a local source of the essential amino acids originated from fibrin clot degradation products.

This is a preview of subscription content, access via your institution.

References

  1. Medved, L. and Nieuwenhuisen, W., Thromb. Haemost., 2003, vol. 89, pp. 409–419.

    CAS  Google Scholar 

  2. Bajzar, L., Nesheim, M., Morser, J., and Tracy, P.B., J. Biol. Chem., 1998, vol. 273, pp. 2792–2798.

    Article  CAS  Google Scholar 

  3. Redlitz, A., Tan, A.K., Eaton, D.L., and Plow, E.F., J. Clin. Invest., 1995, vol. 96, pp. 2534–2538.

    Article  CAS  Google Scholar 

  4. Borgström, A. and Regne’r, S., Pancreatology, 2005, vol. 5, pp. 530–536.

    Article  Google Scholar 

  5. Mosnier, L.O., and Bouma, B.N., Arterioscler. Thromb. Vasc. Biol., 2006, vol. 26, pp. 2445–2453.

    Article  CAS  Google Scholar 

  6. Willemse, J.L. and Hendriks, D.F., Clin. Chem., 2006, vol. 52, pp. 30–36.

    Article  CAS  Google Scholar 

  7. Plow, E.F., Allampallam, K., and Redlitz, A., Trends in Cardiovasc. Med., 1997, vol. 7, no. 3, pp. 71–75.

    Article  CAS  Google Scholar 

  8. Mengerink, Y., Kutlán, D., Tóth F., Csámpai A., and Molnár-Perl, I., J. Chromatogr. A, 2002, vol. 949, pp. 99–124.

    Article  CAS  Google Scholar 

  9. Ceriotti, G. and Spandrio, L., J. Biochem., 1957, vol. 66, pp. 603–607.

    CAS  Google Scholar 

  10. Subbotina, T.F. and Galebskaya, L.V., Biomed. Khim., 2005, vol. 51, pp. 60–65.

    CAS  Google Scholar 

  11. Tietz, N.U., Klinicheskaya otsenka laboratornykh testov (Clinical Guide to Laboratory Tests), Meditsina: Moscow, 1986.

    Google Scholar 

  12. Zhloba, A.A., Regionarnoe krovoobrashchenie i mikrotsirkulyatsiya, 2007, vol. 6, no. 3., pp. 4–14.

    Google Scholar 

  13. Böger, R.H., J. Nutr., 2007, vol. 137, pp.1650S–1655S.

    Google Scholar 

  14. Jin, L., Caldwell, R.B., Li-Masters, T., and Caldwell, R.W., J. Physiol. Pharmacol., 2007, vol. 58, pp. 191–206.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to T. F. Subbotina.

Additional information

Original Russian Text © A.A. Zhloba, T.F. Subbotina, D.S. Lupan, V.A. Bogova, O.A. Kusheleva, 2012, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zhloba, A.A., Subbotina, T.F., Lupan, D.S. et al. Arginine and lysine as products of basic carboxypeptidase activity associated with fibrinolysis. Biochem. Moscow Suppl. Ser. B 6, 261–265 (2012). https://doi.org/10.1134/S1990750812030158

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990750812030158

Keywords

  • fibrinolysis
  • basic carboxypeptidases
  • arginine
  • lysine