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Involvement of integrin α5β1 in apoptosis and drug resistance of human breast carcinoma cells

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Abstract

The doxorubicin-resistant MCF-7Dox cell line derived from the drug-sensitive MCF-7 human breast carcinoma cell line, differs from the latter by a strongly reduced expression of the α2β1 integrin and a highly increased ex-pression of the α5β1 integrin. Silencing of this integrin in the MCF-7Dox cells by transfection with α5-specific siRNA markedly stimulated anoikis and increased sensitivity of these cells to doxorubicin. α5β1 silencing was also accompanied by a significant inhibition of the activity of signal protein kinases Akt and Erk2. Our results suggest involvement of common integrin-mediated signal mechanisms controlling apoptotic cell death under various stress conditions.

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia

    G. E. Morozevich, N. I. Kozlova, N. A. Ushakova, M. E. Preobrazhenskaya & A. E. Berman

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  1. G. E. Morozevich
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  2. N. I. Kozlova
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  3. N. A. Ushakova
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  4. M. E. Preobrazhenskaya
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  5. A. E. Berman
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Correspondence to A. E. Berman.

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Original Russian Text © G.E. Morozevich, N.I. Kozlova, N.A. Ushakova, M.E. Preobrazhenskaya, A.E. Berman, 2011, published in Biomeditsinskaya Khimiya.

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Morozevich, G.E., Kozlova, N.I., Ushakova, N.A. et al. Involvement of integrin α5β1 in apoptosis and drug resistance of human breast carcinoma cells. Biochem. Moscow Suppl. Ser. B 5, 241–245 (2011). https://doi.org/10.1134/S1990750811030085

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  • DOI: https://doi.org/10.1134/S1990750811030085

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