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Anticoagulant and anticomplement effects of an endogenous inhibitor from hepatopancreas of red king crab (Paralithosed camtschaticus) on human blood

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Abstract

Serpins are the superfamily of serine and cysteine protease inhibitors (SERine Protease Inhibitors) acting by an irreversible suicide mechanism. A novel serpin from hepatopancreas of red king crab (Paralithosed camtschaticus) was isolated and its effect on the process of human blood plasma coagulation was investigated. The investigated serpin exhibited a significant anticoagulant effect, which dramatically increased in the combination with heparin. The study of the crab serpin on C1s (C1 esterase) revealed its competition with the C1 inhibitor from blood plasma. Although the inhibitor weakly influenced thrombin activity, inhibition constant for C1s was (2.02 ± 0.71) 10−7 M. Unlike the C1 inhibitor the novel red king crab serpin does not inhibit fibrinolysis but inhibits blood coagulation. This creates certain clinical perspectives.

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References

  1. Isaev, V.A., Rudenskaya, G.N., Rudenskaya, Yu.A., Lyutova, L.V., and Balashova, M.M., Rus. Patent no. 2009143587, 2010.

  2. Silverman, G.A., Bird, P.I., Carrell, R.W., Church, F.C., Coughlin, P.B., Gettins, P.G., Irving, J.A., Lomas, D.A., Luke, C.J., Moyer, R.W., Pemberton, P.A., Remold-O, Donnell, E., Salvesen, G.S., Travis, J., and Whisstock, J.C., J. Biol. Chem., 2001, vol. 276, pp. 33293–33296.

    Article  CAS  Google Scholar 

  3. Law, R.H.P., Zhang, Q., McGowan, Sh., Bucke, A.M., Silverman, G.A., Wong, W., Rosado, C.J., Langendorf, Ch.G., Pike, R.N, Bird, J.C., and Whisstock, R.N., Genome Biology, 2006, vol. 7, pp. 216–225.

    Article  Google Scholar 

  4. Tesch, L.D., Raghavendra, M.P., Bedsted-Faarvang, T., Gettins, P.G., and Olson, S.T., Protein Sci, 2005, vol. 14, pp. 533–542.

    Article  CAS  Google Scholar 

  5. Deutsch, D.G. and Mertz, E.T., Science, 1970, vol. 170, pp. 1095–1096.

    Article  CAS  Google Scholar 

  6. Erlanger, B.F., Kokowsky, N., and Cohen, W., Arch. Biochim. Biophys., 1961, vol. 95, pp. 271–278.

    Article  CAS  Google Scholar 

  7. Stepanov, V.M., Terent’eva, E., Voyushina, T.L., and Gololobov, M., Bioorg. Med. Chem., 1995, vol. 3, pp. 479–485.

    Article  CAS  Google Scholar 

  8. Filippova, I.Yu., Lysogorskaya, E.N., Oksenoit, E.S., Rudenskaya, G.N., and Stepanov, V.M., Anal. Biochem., 1984, vol. 143, pp. 293–297.

    Article  CAS  Google Scholar 

  9. Deutsch, D.G. and Mertz, E.T., Science, 1970, vol. 170, pp. 1095–1096.

    Article  CAS  Google Scholar 

  10. Kozlov, L.V., Shaibanov, B.B., Ivanov, A.E., Zubov, V.P., and Antonov, V.K., Biokhimiya, 1989, vol. 54, pp. 1754–1760.

    Google Scholar 

  11. Andina, S.S., Kozlov, L.V., and D’yakov, V.L., Biomed. Khim., 2004, vol. 50, pp. 86–91.

    CAS  Google Scholar 

  12. Rezaie, A.R., J.Biol.Chem., 1998, vol. 273, pp. 16824–16827.

    Article  CAS  Google Scholar 

  13. Potempa, J., Korzuz, E., and Travis, J., J. Biol. Chem., 1994, vol. 269, pp. 15957–15960.

    CAS  Google Scholar 

  14. Zhou, A., Robin, W.C., and Hungtington, J.A., J. Biol. Chem., 2002, vol. 278, pp. 27541–27547.

    Article  Google Scholar 

  15. Lutz, J., Thurmel, K., and Heemann, U., J. Inflamm. (Lond), 2010, vol. 7, p. 27.

    Article  Google Scholar 

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Authors and Affiliations

  1. Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1/3, Moscow, 119991, Russia

    M. V. Balashova, Yu. A. Rudenskaya, S. S. Andina & G. N. Rudenskaya

  2. Biology Department, “Trinita” Company, Lomonosov Moscow State University, Moscow, Russia

    L. V. Lyutova & V. A. Isayev

  3. Gabrichevskii Research Institute for Microbiology and Epidemiology, Moscow, Russia

    L. V. Kozlov

Authors
  1. M. V. Balashova
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  2. L. V. Lyutova
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  3. Yu. A. Rudenskaya
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  4. V. A. Isayev
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  5. S. S. Andina
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  6. L. V. Kozlov
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  7. G. N. Rudenskaya
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Corresponding author

Correspondence to M. V. Balashova.

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Original Russian Text © M.V. Balashova, L.V. Lyutova, Yu.A. Rudenskaya, V.A. Isayev, S.S. Andina, L.V. Kozlov, G.N. Rudenskaya, 2011, published in Biomeditsinskaya Khimiya.

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Balashova, M.V., Lyutova, L.V., Rudenskaya, Y.A. et al. Anticoagulant and anticomplement effects of an endogenous inhibitor from hepatopancreas of red king crab (Paralithosed camtschaticus) on human blood. Biochem. Moscow Suppl. Ser. B 5, 268–275 (2011). https://doi.org/10.1134/S1990750811030024

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  • DOI: https://doi.org/10.1134/S1990750811030024

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