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Application of the method of measurement of fluorescence intensity in vivo in biological tissues for pharmacokinetic studies of different chlorin-based photosensitizers

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Abstract

A precise and reproducible method of quantitative determination of the photosensitizer (PS) Photolon in liver samples of laboratory animals by means of a spectrophotometric assay with preliminary extraction has been developed. Conditions of the PS extraction have been optimized and validated for the quantitative determination of the PS Photolon by the spectrophotometric assay and the main analytical characteristics have been investigated. Using the method of quantitative determination of the PS Photolon in liver tissue samples a quantitative estimation of in vivo fluorescence of the liver tissue was performed after PS administration to animals. There was a high correlation (R = 0.99) between results obtained by spectrophotometry ex vivo and spectrofluorimetry in vivo. The method of fluorescence detection in vivo is applicable for studies of the pharmacokinetics of different photosensitizers.

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References

  1. Henderson, B.W. and Dougherty, T.J., Photochem. Photobiol., 1992, vol. 55, pp. 145–157.

    Article  CAS  Google Scholar 

  2. Lee, C.C., Pouge, B.W., Strawbridge, R.R., Moodie, K.L., Bartholomew, L.R., Burke, G.C., and Hoopes, P.J., Photochem. Photobiol., 2001, vol. 74, pp. 453–460.

    Article  CAS  Google Scholar 

  3. Cheung, R., Solonenko, M., Bush, T.M., Del Piero, F., Putt, M.E., Hahn, S.M., and Yodh, A.G., J. Biomed. Optic., 2003, vol. 8, pp. 248–252.

    Article  CAS  Google Scholar 

  4. Patterson, M.S., Wilson, B.C., Feather, J.W., Burns, D.M., and Pushka, W., Photochem. Photobiol., 1987, vol. 46, pp. 337–343.

    Article  CAS  Google Scholar 

  5. Bourré, L., Thibaut, S., Briffaud, A., Rousset, N., Eléouet, S., Lajat, Y., and Patrice, T., Photochem. Photobiol. B., 2002, vol. 67, pp. 23–31.

    Article  Google Scholar 

  6. Thibaut, S., Bourre, L., Bendarraz, A., Juillard, S., Simonneaux, G., Lajat, Y., and Patrice, T., J. Photodiagnosis and Photodynamic Therapy, 2004, vol. 1, pp. 181–190.

    CAS  Google Scholar 

  7. Kasselouri, A., Bourdon, O., Demore, D., Blais, J.C., Prognon, P., Bourg-Heckly, G., and Blais, J., Photochem. Photobiol., 1999, vol. 70, pp. 275–279.

    Article  CAS  Google Scholar 

  8. Lilge, L., O’Carroll, C., and Wilson, B.C., Photochem. Photobiol. B., 1997, vol. 39, pp. 229–235.

    Article  CAS  Google Scholar 

  9. Glanzmann, T., Hadjur, C., Zellweger, M., Grosiean, P., Forrer, M., Ballini, J., Monnier, P., van den Bergh, H., Lim, C., and Wagnieres, G., Photochem. Photobiol., 1998, vol. 67, pp. 596–602.

    CAS  Google Scholar 

  10. Panjehpour, M., Sneed, R.E., Frazier, D.L., Barnhill, M.A., O’Brien, S.F., Harb, W., and Overholt, B.F., J. Lasers Surg. Med., 1993, vol. 13, pp. 23–30.

    Article  CAS  Google Scholar 

  11. Stepp, H., Beck, T., Beyer, W., Pfaller, C., Schuppler, M., Sroka, R., and Baumgartner, R., Med. Laser Appl., 2007, vol. 22, pp. 23–34.

    Article  Google Scholar 

  12. Isakau, H.A., Trukhacheva, T.V., Zhebentyaev, A.I., and Petrov, P.T., J. Biomed. Chromatogr., 2007, vol. 21, pp. 318–325.

    Article  CAS  Google Scholar 

  13. Isakau, H.A., Trukhacheva, T.V., and Petrov, P.T., J. Pharmaceut. Biochem. Analys., 2007, vol. 45, pp. 20–29.

    Article  CAS  Google Scholar 

  14. Moan, J., Ma, L.W., Juzeniene, A., Iani, V., Juzenas, P., Apricena, F., Teng, Q., Int. J. Cancer, 2003, vol. 103, pp. 132–135.

    Article  CAS  Google Scholar 

  15. Wang, Z.J., He, Y.Y., Huang, C.G., Huang, J.S., Huang, Y.C., An, J.Y., et al., Photochem. Photobiol., 1999, vol. 70, pp. 773–780.

    Article  CAS  Google Scholar 

  16. Shah, V.P., Guidance for Industry: Bioanalytical Methods Validation, Rockville: US Department of Health and Human Services, Food and Drug Administration, CDER, 2001.

    Google Scholar 

  17. Mang, T., Kost, J., Sullivan, M., and Wilson, B.C., J. Photodiagnosis and Photodynamic Therapy, 2006, vol. 3, pp. 168–176.

    Article  CAS  Google Scholar 

  18. Melo, C.A.S., Kurachi, C., Grecco, C., Sibata, C.H., Castro-e-Silva, O., and Bagnato, V.S., J. Photochem.Photobiol. B., 2004, vol. 73, pp. 183–188.

    Article  CAS  Google Scholar 

  19. Kennedy, J.C., Nadeau, P., Petryka, Z.J., Pottier, R.H., and Weagle, G., Photochem. Photobiol., 1992, vol. 55, pp. 729–734.

    Article  CAS  Google Scholar 

  20. Kessel, D., Photochem. Photobiol. B., 1997, vol. 39, pp. 81–83.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. RUE Belmedpreparaty, ul. Fabritsyusa 30, Minsk, 220007, Republic of Belarus

    S. V. Shlyakhtin, T. V. Trukhacheva & G. A. Isakov

  2. National Center of Oncology and Medical Radiology, Minsk, Republic of Belarus

    Yu. P. Istomin

Authors
  1. S. V. Shlyakhtin
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  2. T. V. Trukhacheva
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  3. G. A. Isakov
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  4. Yu. P. Istomin
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Correspondence to S. V. Shlyakhtin.

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Original Russian Text © S.V. Shlyakhtin, T.V. Trukhacheva, G.A. Isakov, Yu.P. Istomin, 2010, published in Biomeditsinskaya Khimiya.

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Shlyakhtin, S.V., Trukhacheva, T.V., Isakov, G.A. et al. Application of the method of measurement of fluorescence intensity in vivo in biological tissues for pharmacokinetic studies of different chlorin-based photosensitizers. Biochem. Moscow Suppl. Ser. B 4, 184–190 (2010). https://doi.org/10.1134/S1990750810020095

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  • DOI: https://doi.org/10.1134/S1990750810020095

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