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Phosphorylation of ATM/ATR substrates in eukaryotic cells after infection with Helicobacter pylori

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Abstract

Phosphorylation of ATM-kinase substrates in HeLa and AGS cells in response to Helicobacter pylori infection has been characterized. Infection with wild-type (cagPAI-positive) and corresponding isogenic cagPAI negative mutant induced activation of Chk1 and Chk2 kinases. However, only Chk1 was directly activated by ATM-kinase. Using 2D-electrophoresis and mass spectrometry a group of proteins phosphorylated in AGS cells by ATM1/ATR kinases during H. pylori infection has been identified.

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Authors and Affiliations

  1. Department of Microbiology, Kazan State University, ul. Kremlevskaya 18, Kazan, 420008, Russia

    M. O. Anikeenok & O. N. Ilinskaya

  2. Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany

    Yu. N. Churin & T. F. Meyer

Authors
  1. M. O. Anikeenok
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  2. Yu. N. Churin
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  3. T. F. Meyer
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  4. O. N. Ilinskaya
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Correspondence to M. O. Anikeenok.

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Original Russian Text © M.O. Anikeenok, Yu.N. Churin, T.F. Meyer, O.N. Ilinskaya, 2010, published in Biomeditsinskaya Khimiya.

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Anikeenok, M.O., Churin, Y.N., Meyer, T.F. et al. Phosphorylation of ATM/ATR substrates in eukaryotic cells after infection with Helicobacter pylori . Biochem. Moscow Suppl. Ser. B 4, 171–176 (2010). https://doi.org/10.1134/S1990750810020071

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  • DOI: https://doi.org/10.1134/S1990750810020071

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