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Mass spectrometry label-free quantitative analysis of proteins

  • Proteomics and Bioinformatics
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Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

The dependence of the intensity of a mass spectrometric signal on protein concentration has been investigated using individual purified proteins and complex protein mixtures. Linearity of this dependence has been determined within the concentration ranges from 1 nM to 1 μM. In this range the dependence of the mass spectrometric signal intensity on concentration possesses a slope (tangent) characteristic for each protein studied. The efficacy of the method has been evaluated using the other methods (AQUA and 18O-labelling) employed in quantitative proteomics.

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya10, Moscow, 119121, Russia

    A. T. Kopylov, V. G. Zgoda & A. I. Archakov

Authors
  1. A. T. Kopylov
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  2. V. G. Zgoda
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  3. A. I. Archakov
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Correspondence to A. T. Kopylov.

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Original Russian Text © A.T. Kopylov, V.G. Zgoda, A.I. Archakov, 2010, published in Biomeditsinskaya Khimiya.

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Kopylov, A.T., Zgoda, V.G. & Archakov, A.I. Mass spectrometry label-free quantitative analysis of proteins. Biochem. Moscow Suppl. Ser. B 4, 49–58 (2010). https://doi.org/10.1134/S1990750810010075

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  • DOI: https://doi.org/10.1134/S1990750810010075

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