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Visualization and identification of hepatitis C viral particles by atomic force microscopy combined with MS/MS analysis

  • Nanobiotechnologies in Medicine
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Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

A possibility of detection and identification of hepatitis C viral (HCV) particles by atomic force microscopy (AFM) in combination with mass spectrometry (MS) has been investigated. The AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on the surface of a AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto the surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed on AFM-nanochips in quantity sufficient for their subsequent mass spectrometric identification. Thus, the AFM/MS approach allows to identify fragments of hepatitis C virus fished on the surface of AFM-nanochip from serum.

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References

  1. Kato, N., Microb. Comp. Genomics., 2000, vol. 5, pp. 129–151.

    CAS  Google Scholar 

  2. Simmonds, E., Hepatology, 1994, vol. 19, pp. 1321–1324.

    Article  CAS  Google Scholar 

  3. Choo, Q.L., Kuo, G., and Weiner, A.J., Science, 1989, vol. 244, pp. 359–362.

    Article  CAS  Google Scholar 

  4. Ogata, N., Alter, H.J., Miller, R.H., and Purcell, R.H., Proc. Natl. Acad. Sci. USA, 1991, vol. 88, pp. 3392–3396.

    Article  CAS  Google Scholar 

  5. Alberti, A.L., Hepatology, 2002, vol. 36, pp. 195–200.

    Article  Google Scholar 

  6. Di Bisceglie, A.M., Hepatology, 2000, vol. 31, pp. 1014–1018.

    Article  Google Scholar 

  7. Seeff, L.B., Hepatology, 1997, vol. 26, pp. 21–28.

    Article  Google Scholar 

  8. Archakov, A.I., Ivanov, Y.D., Lisitsa, A.V., and Zgoda, V.G., Proteomics, 2007, vol. 7, pp. 4–9.

    Article  CAS  Google Scholar 

  9. Ivanov, Yu. D., Kanaeva, I.P., Eldarov, M.A., Skryabin, K.G., Lehnerer, M., Schulze, J., Hlavica, P., Archakov, A.I., Biochem. Mol. Biol. Int., 1997, vol. 42, pp. 731–737.

    CAS  Google Scholar 

  10. Ivanov, Yu. D., Kanaeva, I.P., Kuznetsov, V.Yu., Lehnerer, M., Schulze, J., Hlavica, P., and Archakov, A.I., Arch. Biochem. Biophys., 1999, vol. 362, pp. 87–93.

    Article  CAS  Google Scholar 

  11. Ivanov, Yu.D., Usanov, S.A., and Archakov, A.I., Biochem. Mol. Biol. Int., 1999, vol. 47, pp. 327–336.

    CAS  Google Scholar 

  12. Kuznetsov, V.Yu., Ivanov, Yu.D., and Archakov, A.I., Proteomics, 2004, vol. 4, pp. 2390–2396.

    Article  CAS  Google Scholar 

  13. Kuznetsov, V.Yu., Ivanov, Y.D., Bykov, V.A., Saunin, S.A., Fedorov, I.A., Lemeshko, S.V., Hui Bon Hoa, G., and Archakov, A.I., Proteomics, 2002, vol. 2, pp. 1699–1705.

    Article  CAS  Google Scholar 

  14. Kiselyova, O.I., Yaminsky, I.V., Ivanov, Yu.D., Kanaeva, I.P., Kuznetsov, V.Yu., and Archakov, A.I., Arch. Biochem. Biophys., 1999, vol. 371, pp. 1–7.

    Article  CAS  Google Scholar 

  15. Ivanov, Yu.D., Kanaeva, I.P., and Archakov, A.I., Biochem. Biophys. Res. Commun., 2000, vol. 273, pp. 750–752.

    Article  CAS  Google Scholar 

  16. Ivanov, Yu.D., Kanaeva, I.P., Gnedenko, O.V., Pozdnev, V.F., Shumyantseva, V.V., Samenkova, N.F., Kuznetsova, G.P., Tereza, A.M., Schmid, R.D., and Archakov, A.I. J. Mol. Recognition, 2001, vol. 14, pp. 185–196.

    Article  CAS  Google Scholar 

  17. Semenova, N.V., Ivanov, Yu.D., Gnedenko, O.V., Konev, V.A., Kovalev, O.B., Uchaikin, V.F., Morozov, S.G., and Archakov, A.I., Allergiya, Astma, i Klinicheskaya Immunologiya, 2001, vol. 1., pp. 183–185.

    Google Scholar 

  18. Ivanov, Yu.D., Gnedenko, O.V., Konev, V.A., Kovalev, O.B., Nikolaeva, L.I., Semenova, N.V., Uchaikin, V.F., and Archakov, A.I., Vopr. Med. Khim., 2001, vol. 47, pp. 419–425.

    CAS  Google Scholar 

  19. Dmitriev, D.A., Massino, Yu.S., Segal, O.L., Smirnova, M.B., Pavlova, E.V., Kolyaskina, G.I., Gurevich, K.G., Gnedenko, O.V., Ivanov, Yu.D., Archakov, A.I., Osipov, A.P., Dmitriev, A.D., and Egorov, A.M., Biochemistry (Moscow), 2002, vol. 67, pp. 1643–1654.

    Article  Google Scholar 

  20. Dmitriev, D.A., Massino, Y.S., Segal, O.L., Smirnova, M.B., Pavlova, E.V., Gurevich, K.G., Gnedenko, O.V., Ivanov, Y.D., Kolyaskina, G.I., Archakov, A.I., Osipov, A.P., Dmitriev, A.D., and Egorov, A.M., J. Immunol. Methods, 2002, vol. 261, pp. 103–118.

    Article  CAS  Google Scholar 

  21. Archakov, A.I. and Ivanov, Y.D., Methods Enzymol., 2002, vol. 357, pp. 94–103.

    Article  CAS  Google Scholar 

  22. Veselovsky, A.V., Ivanov, Yu.D., Ivanov, A.S., Archakov, A.I., Lewi, P., and Janssen, P., J. Mol. Recognition, 2002, vol. 15, pp. 405–422.

    Article  CAS  Google Scholar 

  23. Archakov, A.I., Ivanov, Yu.D., Govorun, V.M., Uchaikin, V.F., Nikolaeva, L.I., Gnedenko, O.V., Konstantinova, N.I., Kovalev, A.V., and Konev, V.A., Allergiya, Astma i Immunologiya, 2003, vol. 9, pp. 175–178.

    Google Scholar 

  24. Ivanov, Yu.D., Gnedenko, O.V., Nikolaeva, L.I., Konev, V.A., Kovalev, O.B., Uchaikin, V.F., Govorun, V.M., Pokrovskii, V.I., and Archakov, A.I., Zhurn. Microbiol., 2003, vol. 2, pp. 58–62.

    Google Scholar 

  25. Archakov, A.I., Govorun, V.M., Dubanov, A.V., Ivanov, Yu.D., Veselovsky, A.V., Lewi, P., and Janssen, P., Proteomics, 2003, vol. 3, pp. 380–391.

    Article  CAS  Google Scholar 

  26. Ivanov, Yu.D., Govorun, V.M., Bykov, V.A., and Archakov, A.I., Proteomics, 2006, vol. 6, pp. 1399–1414.

    Article  CAS  Google Scholar 

  27. Archakov, A.I., and Ivanov, Yu.D., Molecular Biosystems, 2007, vol. 3, pp. 336–342.

    Article  CAS  Google Scholar 

  28. Medvedeva, N.V., Ipatova, O.M., Ivanov, Yu.D., Drozhzhin, A.I., and Archakov A.I., Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2007, vol. 1, pp. 114–124.

    Article  Google Scholar 

  29. Ivanov, Yu.D., Uchaikin, V.F., Pleshakova, T.O., Frantsuzov, P.A., Svetlov, S.K., Konev, V.A., Kovalev, O.B., Yastrebova, O.N., Sveshnikov, P.G., Ulanova, T.I., and Archakov, A.I., Phiziologiya i Patologiya Immunnoi Sistemy, 2006, vol. 12, pp. 11–14.

    Google Scholar 

  30. Archakov, A., Ivanov, Y., Lisitsa, A., Zgoda, V., Proteomics, 2009, vol. 9, pp. 1326–1343.

    Article  CAS  Google Scholar 

  31. Ivanov, Yu.D., Frantsuzov, P.A., Pleshakova, T.O., Ziborov, V.S., Svetlov, S.K., Krokhin, N.V., Konev, V.A., Kovalev, O.B., Uchaikin, V.F., Yastrebova, O.N., Sveshnikov, P.G., and Archakov, A.I., Biomed. Khim., 2009, vol. 55, pp. 689–701.

    Google Scholar 

  32. Shevchenko, A., Keller, P., Mann, M., and Simons, K., Electrophoresis, 1997, vol. 18, pp. 2591–2600.

    Article  CAS  Google Scholar 

  33. Kussmann, M., and Roepstorf, P., Methods in Molecular Biology, 2000, vol. 146, pp. 405–424.

    CAS  Google Scholar 

  34. Bukh, J., Purcell, R.H., and Miller, R.H., Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 8239–8243.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia

    A. L. Kaysheva, Yu. D. Ivanov, V. G. Zgoda, P. A. Frantsuzov, T. O. Pleshakova, N. V. Krokhin & A. I. Archakov

  2. Joint Institute for High Temperatures of Russian Academy of Sciences, Moscow, Russia

    V. S. Ziborov

Authors
  1. A. L. Kaysheva
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  2. Yu. D. Ivanov
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  3. V. G. Zgoda
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  4. P. A. Frantsuzov
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  5. T. O. Pleshakova
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  6. N. V. Krokhin
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  7. V. S. Ziborov
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  8. A. I. Archakov
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Correspondence to A. L. Kaysheva.

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Original Russian Text © A.L. Kaysheva, Yu.D. Ivanov, V.G. Zgoda, P.A. Frantsuzov, T.O. Pleshakova, N.V. Krokhin, V.S. Ziborov, A.I. Archakov, 2010, published in Biomeditsinskaya Khimiya.

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Kaysheva, A.L., Ivanov, Y.D., Zgoda, V.G. et al. Visualization and identification of hepatitis C viral particles by atomic force microscopy combined with MS/MS analysis. Biochem. Moscow Suppl. Ser. B 4, 15–24 (2010). https://doi.org/10.1134/S1990750810010038

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  • DOI: https://doi.org/10.1134/S1990750810010038

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