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Variations of glycoprotein IIb–IIIa (αIIb/β3-integrin) content in healthy donors. The influence on platelet aggregation activity and efficacy of aspirin action

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Abstract

The effects of content of a fibrinogen receptor, glycoprotein (GP) IIb–IIIa (αIIb/β3-integrin), GP IIIa genetic polymorphism (substitution Leu33Pro), and fibrinogen concentration in blood plasma on platelet aggregation activity have been investigated in a group of healthy volunteers. In 35 examined donors the GP IIb–IIIa content on platelet surface varied from 40 to 71 × 103 per platelet. Repeated measurements revealed that the GP IIb–IIIa content coefficient of variation was 9.5%, and deviations from mean levels did not exceed 20%. The level and the rate of platelet aggregation induced by ADP (1.25–20 μM) correlated with GP IIb–IIIa number (r from 0.315 to 0.591) and were higher in the group of donors with high in comparison with low GP IIb–IIIa content (>60 and (40–50) × 10−3 per platelet, respectively). Aspirin, the inhibitor of thromboxane A2 synthesis, partially suppressed ADP-induced platelet aggregation. The level of residual aggregation in the presence of aspirin also correlated with GP IIb–IIIa content and increased in subjects with high receptor content. Parameters of ADP-induced aggregation did not differ in donors with genotypes GP IIIa Pro33(−) (Leu33Leu33, n = 20) and Pro33(+) (Leu33Pro33, n = 13, and Pro33Pro33, n = 2) genotype. GP IIb–IIIa content was also not affected by GP IIIa polymorphism. No significant correlations were found between the level and rate of platelet aggregation and fibrinogen concentration in blood plasma. The data obtained indicate that the effects of variations of GP IIb–IIIa content on platelet aggregation are higher than GP IIIa Leu33Pro polymorphism and variations of fibrinogen concentration. High GP IIb–IIIa content is associated with increased platelet aggregation activity and decreased efficacy of aggregation inhibition by aspirin.

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Authors and Affiliations

  1. Cardiology Research and Production Complex, Rosmedtechnology, ul. 3-ya Cherepkovskaya 15a, Moscow, 121552, Russia

    S. G. Khaspekova, Y. V. Shimanova & A. V. Mazurov

  2. Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Leningrad region, Gatchina, 188350, Russia

    O. V. Sirotkina

Authors
  1. S. G. Khaspekova
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  2. O. V. Sirotkina
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  3. Y. V. Shimanova
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  4. A. V. Mazurov
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Correspondence to A. V. Mazurov.

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Original Russian Text © S.G. Khaspekova, O.V. Sirotkina, Y.V. Shimanova, A.V. Mazurov, 2009, published in Biomeditsinskaya Khimiya.

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Khaspekova, S.G., Sirotkina, O.V., Shimanova, Y.V. et al. Variations of glycoprotein IIb–IIIa (αIIb/β3-integrin) content in healthy donors. The influence on platelet aggregation activity and efficacy of aspirin action. Biochem. Moscow Suppl. Ser. B 3, 92–98 (2009). https://doi.org/10.1134/S1990750809010144

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  • DOI: https://doi.org/10.1134/S1990750809010144

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