Skip to main content
SpringerLink
Log in

Ribosomal repeat in cell free DNA as a marker for cell death

  • Clinical Studies
  • Published:
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

A novel method for in vivo evaluation of cell death in patients with acute and/or chronic heart diseases, accompanied by apoptosis or cell necrosis has been developed. The method is based on the analysis of cell free DNA (cfDNA) in the blood serum (plasma). It includes estimation of concentration of serum ribosomal repeat (rDNA), content of rDNA in total cfDNA, as well as factors of cfDNA elimination, such as nuclease activity and anti-DNA antibody. We have found a fivefold increase in serum cfDNA concentration and a 12-fold increase of serum rDNA concentration in patients with acute myocardial infarction compared with healthy individuals. In chronic coronary ischemia serum cfDNA concentration was nearly normal, but the content of rDNA in cfDNA was 4.8-fold higher, and the serum rDNA concentration was increased sevenfold. We hypothesize that putative reason for accumulation of rDNA within cfDNA might be attributed to the previously reported resistance of rDNA to the ds-fragmentation by serum endonucleases. In acute and chronic coronary disease serum nuclease activity and the titer of anti-DNA antibodies (which are mainly bound to the cfDNA) was substantially higher than in the healthy controls. It is suggested that release of rDNA fragments into blood not only reflects cellular death in the body but also determines the response of the organism to the disease-associated stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vlassov, V.V., Laktionov, P.P., and Rykova, E.Y., Bioessays, 2007, vol. 29, pp. 654–667.

    Article  CAS  Google Scholar 

  2. Antonatos, D., Patsilinakos, S., Spanodimos, S., Korkonikitas, P., and Tsigas, D., Ann. N.Y. Acad. Sci., 2006, vol. 1075, pp. 278–281.

    Article  CAS  Google Scholar 

  3. Kawai, Y., Yoshida, M., Arakawa, K., Kumamoto, T., Morikawa, N., Masamura, K., Tada, H., Ito, S., Hoshizaki, H., Oshima, S., Taniguchi, K., Terasawa, H., Miyamori, I., Kishi, K., and Yasuda, T., Circulation, 2004, vol. 109, pp. 2398–2400.

    Article  CAS  Google Scholar 

  4. Veiko, N.N., Kostyuk, S.V., Ermakov, A.V., Kalashnikova, E.A., Ryazantseva, T.A., and Speranskii, A.I., Byull. Eksper. Biol. Med., 2007, vol. 144, pp. 277–282.

    Google Scholar 

  5. Veiko, N.N. and Spitkovskii, D.M., Radiatsionnaya Biologiya. Radioekologiya, 2000, vol. 40, pp. 396–404.

    CAS  Google Scholar 

  6. Veiko, N.N., Shubaeva, N.O., Ivanova, S.M., Speranskii, A.I., Lyapunova, N.A., and Spitkovskii, D.M., Byull. Eksper. Biol. Med., 2006, vol. 143, pp. 282–285.

    Google Scholar 

  7. Veiko, N.N., Egolina, N.A., Radzivil, G.G., Nurbaev, S.D., Kosyakova, N.V., Shubaeva, N.O., and Lyapunova, N.A., Mol. Biol., 2003, vol. 37, pp. 409–419.

    Article  CAS  Google Scholar 

  8. Veiko, N.N., Lyapunova, N.A., Bogush, A.I., Tsvetkova, T.G., and Gromova, E.V., Mol. Biol., 1996, vol. 30, pp. 1076–1084.

    CAS  Google Scholar 

  9. Ermakov, A.V., Kostyuk, S.V., Egolina, N.A., Malinovskaya, E.M., and Veiko, N.N., Radioatsionnaya Biologiya. Radioekologiya, 2007, vol. 47, pp. 133–140.

    CAS  Google Scholar 

  10. Pisetsky, D.S. and Gonzalez, T.C., clin. Exp. Immunol., 1999, vol. 116, pp 354–359.

    Article  CAS  Google Scholar 

  11. Vernon, S.D., Shukla, S.K., Conradt, J., Unger, E.R., and Reeves, W.C., BMC Microbiol., 2002, vol. 2, pp. 2–39.

    Article  Google Scholar 

  12. Stroun, M., Lyautey, J., Lederrey, C., Mulcahy, H.E., and Anker, P., Ann. N.Y. Acad. Sci., 2001, vol. 945, pp. 258–264.

    Article  CAS  Google Scholar 

  13. Yasutomo, K., Horiuchi, T., Kagami, S., Tsukamoto, H., Hashimura, C., Urushihara M., and Kuroda, Y., Nat. Genet., 2001, vol. 28, pp. 313–314.

    Article  CAS  Google Scholar 

  14. Napirei, M., Karsunky, H., Zevnik, B., Stephan, H., Mannherz, H.G., and Möröy, T., Nat. Genet., 2000, vol. 25, pp. 135–136.

    Article  CAS  Google Scholar 

  15. Kostyuk, S.V., Fragmenty ribosomnykh genov v sostave vnekletochnoi DNK-factory stress-signalizatsii, Cand. Sci. Dissertation: Research Center for Medical Genetics RAMS, Moscow, 2007.

    Google Scholar 

  16. Kyung, Y., Tuetken, R., Redford, T., MaWaldschmidt, R., Kirsch, J., and Krieg, A.M., J. Immunol., 1998, vol. 160, pp. 4755–4761.

    Google Scholar 

  17. Klinman, D.M., Yi, A.K., Beaucage, S.L., Conover, J., and Krieg, A.M., Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 879–883.

    Article  Google Scholar 

  18. Veiko, N.N., Kalashnikova, E.A., Kokarovtseva, S.N., Kostuk, S.V., Ermakov, A.V., Ivanova, S.M., Ryazantseva, T.A., Egolina, N.A., Lyapunova, N.A., and Spitkovskii, D.M., Byull. Eksper. Biol. Med., 2006, vol. 143, pp. 410–414.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Center for Medical Genetics, Russian Academy of Medical Sciences, ul. Moskvorechie 1, Moscow, 115478, Russia

    N. N. Veiko, N. A. Bulycheva, R. V. Veiko & E. S. Ershova

  2. Central Clinical Hospital, Russian Academy of Sciences, Litovskyi bulv. 1a, Moscow, 117593, Russia

    O. A. Roginko, O. A. Kozdoba & A. A. Yudin

  3. Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia

    V. A. Kuzmin & A. M. Vinogradov

  4. Institute of Rheumatology of Russian Academy of Medical Sciences, Kaschirskoe sh. 34a, Moscow, 115522, Russia

    A. I. Speranskyi

Authors
  1. N. N. Veiko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. A. Bulycheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. A. Roginko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. V. Veiko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. S. Ershova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. O. A. Kozdoba
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. A. Kuzmin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. M. Vinogradov
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. A. Yudin
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. A. I. Speranskyi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. N. Veiko.

Additional information

Original Russian Text © N.N. Veiko, N.A. Bulycheva, O.A. Roginko, R.V. Veiko, E.S. Ershova, O.A. Kozdoba, V.A. Kuzmin, A.M. Vinogradov, A.A. Yudin, A.I. Speranskyi, 2008, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Veiko, N.N., Bulycheva, N.A., Roginko, O.A. et al. Ribosomal repeat in cell free DNA as a marker for cell death. Biochem. Moscow Suppl. Ser. B 2, 198–207 (2008). https://doi.org/10.1134/S1990750808020121

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990750808020121

Key words

Search

Navigation