Abstract
To clarify the role of chaperones in the development of amyloid diseases, the interaction of the chaperonin GroEL with misfolded proteins and recombinant prions has been studied. The efficiency of the chaperonin-assisted folding of denatured glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was shown to be decreased in the presence of prions. Prions are capable of binding to GroEL immobilized on Sepharose, but this does not prevent the interaction between GroEL and other denatured proteins. The size of individual proteins (GroEL, GAPDH, and the recombinant prion) and aggregates formed after their mixing have been determined by the dynamic light scattering analysis. It was shown that at 25°C, the non-functioning chaperonin (equimolar mixture of GroEL and GroES in the absence of Mg-ATP) bound prion yielding large aggregates (greater than 400 nm). The addition of Mg-ATP decreased significantly the size of the aggregates to 70–80 nm. After blocking of one of the chaperonin active sites by oxidized denatured GAPDH, the aggregate size increased to 1200 nm, and the addition of Mg-ATP did not prevent the aggregation. These data indicate the significant role of chaperonins in the formation of amyloid structures and demonstrate the acceleration of aggregation in the presence of functionally inactive chaperonins. The suggested model can be used for the analysis of the efficiency of antiaggregants in the system containing chaperonins.
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Abbreviations
- GAPDH:
-
glyceraldehyde-3-phosphate dehydrogenase
- GdHCl:
-
guanidine hydrochloride
- GroEL and GroES:
-
bacterial chaperonin and co-chaperonin, respectively
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Original Russian Text © I.N. Naletova, E.V. Schmalhausen, I.N. Shalova, A.P. Pleten’, K. Tsiroulnikov, T. Haertle, V.I. Muronetz, 2007, published in Biomeditsinskaya Khimiya.
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Naletova, I.N., Schmalhausen, E.V., Shalova, I.N. et al. Non-functioning chaperonin GroEL stimulates protein aggregation. Biochem. Moscow Suppl. Ser. B 1, 160–163 (2007). https://doi.org/10.1134/S1990750807020096
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DOI: https://doi.org/10.1134/S1990750807020096