Abstract
Thrombin receptor agonist peptide (TRAP-6) may be successfully used instead of thrombin to stimulate regeneration of damaged tissues. Thrombin application is limited by its high price, instability, and proin-flammatory effect at high concentrations. Immobilization of TRAP-6 into a matrix based on lactic and glycolic acid copolymer (PLGA) prevents its destruction by peptidases located in the wound and can also provide controlled release of the peptide. PLGA microparticles with the immobilized peptide were prepared by the double emulgation method. The presence of the immobilized peptide increased the porosity of the microparticle surface detected by scanning electron microscopy. Kinetics of the TRAP-6 release was characterized by a dramatic increase in its concentration in buffer solution (pH 7.5) during the first 2 h after the experiment beginning, and the complete release of the peptide after 20 h. An investigation of TRAP-6 destruction by scanning electron microscopy revealed the increase in the microparticle size and surface porosity already after one day of incubation, and the destroyed microparticles were aggregated by the seventh day of the incubation. Thus, peptide immobilization into PLGA microparticles may be employed for elaboration of a prolonged action preparation with the controlled release of the active agent (peptide).
Similar content being viewed by others
References
Regan, M., Barbul, A., Schlag, G., and Redl, H., Eds., Wound Healing, New York: Springer Verlag, 1994.
Richardson, T., Petrs, M., Ennett, A., and Mooney, D., Nature Biotechnol., 2001, vol. 19, pp. 1029–1034.
Shirakata, Y., Kimura, R., Nanba, D., et al., J. Cell Sci., 2005, vol. 118, Pt. 11, pp. 2363–2370.
Tanaka, A., Nagate, T., and Matsuda, H., J. Vet. Med. Sci., 2005, vol. 67, pp. 909–913.
Strukova, S.M., Dugunova, T.N., Chistov, I.V., and Markvicheva, E.A., Bioorg. Khim., 1998, vol. 24, pp. 256–259.
Surazynski, A., Sienkiewicz, P., Wolczynski, S., and Polka, J., Pharmacol. Res., 2005, vol. 51, pp. 217–221.
Vu, T.K., Hung, D.T., Wheaton, V.I., and Coughlin, S.R., Cell, 1991, vol. 64, pp. 1057–1068.
Van Obberghen-Schilling, E., Rasmussen, U.B., Vouret-Craviari, V., et al., Biochem. J., 1993, vol. 292, pp. 667–671.
Vouret-Craviari, V., Van Obberghen-Schilling, E., and Scimeca, J.C., Biochem. J., 1993, vol. 289, pp. 209–214.
Strukova, S.M., Dugina, T.N., Chistov, I.V., et al., Clin. Appl. Thromb. Hemost., 2001, vol. 7, pp. 325–329.
Nihant, N., Shugens, Ch., Grandfils, Ch., et al., Pharm. Res., 1994, vol. 11, pp. 1479–1484.
Nihant, N., Schugens, Ch., Grandfils, Ch., et al., J. Colloid Interface Sci., 1995, vol. 173, pp. 55–65.
Mac, A., Negi, D., and Friend, D., J. Microencapsulation, 1989, vol. 6, pp. 361–367.
Ogawa, Y., Okada, H., Yamamoto, M., and Shimamoto, T., Chem. Pharm. Bull., 1988, vol. 36, pp. 1502–1507.
Jain, R., Shah, N.H., Malick, A.W., and Rhodes, C.T., Drug. Dev. Ind. Pharm., 1998, vol. 24, pp. 703–727.
Pitt, G., Chasalow, F.I., Hibionada, Y.M., et al., J. Appl. Polym. Sci., 1981, vol. 26, pp. 3779–3787.
Kawaguchi, T., Nakano, M., Juni, K., et al., Chem. Pharm. Bull., 1982, vol. 30, pp. 1517–1520.
Bogdansky, S., in Biodegradable Polymers as Drug Delivery Systems, Chasin, M. and Langer, R., Eds., New York: Marcel Dekker, 1990, pp. 231–259.
Leong, K.W., Damore, P., Marietta, M., and Langer, R., J. Biomed. Mater. Res., 1986, vol. 20, pp. 51–64.
Shea, L.D., Wang, D., Franceschi, R.T., and Mooney, D.J., Tissue Eng., 2000, vol. 6, no. 6, pp. 605–617.
Hollinger, J.O. and Schmitz, J.P., J. Oral Maxilofac. Surg., 1987, vol. 45, pp. 594–600.
Mayer, J., Karamuk, E., Akaike, T., and Wintermantel, E., J. Control. Release, 2000, vol. 64, nos. 1–3, pp. 81–90.
Widmer, M.S., Gupta, P.K., Lu, L., et al., Biomaterials, 1998, vol. 19, pp. 1945–1955.
Agrawal, C.M., and Ray, R.B., J. Biomed. Mater. Res., 2001, vol. 55, no. 2, pp. 141–150.
Mikhailova, A.G., Likhareva, V.V., Vaskovskii, B.V., et al., Biochemistry (Moscow), 2004, vol. 69, pp. 909–917.
Dhanaraju, M.D., Jayakumar, R., and Vamsadhara, C., Chem. Pharm. Bull. (Tokyo), 2004, vol. 52, no. 8, pp. 976–979.
Huang, Y.Y. and Chung, T.W., J. Microencapsul., 2001, vol. 18, pp. 457–465.
Sendil, D., Gursel, I., Wise, D.L., and Hasirci, V., J. Control Release, 1999, vol. 59, no. 2, pp. 207–217.
Hussain, M., Beale, G., Hughes, M., and Akhtar, S., Int. J. Pharm., 2002, vol. 234, nos. 1–2, pp. 129–138.
Fukushima, S., Nishida, M., and Nakano, M., Chem. Pharm. Bull. (Tokyo), 1987, vol. 35, pp. 3375–3381.
Singh, M., Li, X.M., McGee, J.P., et al., Vaccine, 1997, vol. 15, pp. 475–481.
Kumanohoso, T., Natsugoe, S., Shimada, M., and Aikou, T., Cancer Chemother. Pharmacol., 1997, vol. 40, pp. 112–116.
Porjazoska, A., Goracinova, K., Mladenovska, K., et al., Acta Pharm., 2004, vol. 54, pp. 215–229.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © K.S. Stashevskaya, E.A. Markvicheva, S.M. Strukova, A.V. Rusanova, A.M. Makarova, L.R. Gorbacheva, I.A. Prudchenko, V.P. Zubov, K. Grandfis, 2007, published in Biomeditsinskaya Khimiya.
Rights and permissions
About this article
Cite this article
Stashevskaya, K.S., Markvicheva, E.A., Strukova, S.M. et al. Biodegradable microparticles with immobilized peptide for wound healing. Biochem. Moscow Suppl. Ser. B 1, 147–154 (2007). https://doi.org/10.1134/S1990750807020072
Received:
Issue Date:
DOI: https://doi.org/10.1134/S1990750807020072