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Influence of Low-Intensive He-Ne Laser Radiation on the Composition and Content of Phospholipids and Sterols in the Callus Tissues of Wheat Тriticum aestivum L.

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

The effect of He-Ne laser radiation on the composition and content of cell membrane components, namely phospholipids (PL) and sterols, in wheat callus tissues was studied by chromato-mass spectrometry and thin-layer chromatography. It was shown that irradiation of calluses with laser light at a dose of 3.6 J/cm2 resulted in significant changes in the content of these components. Thus, the content of phosphatidylinositol increased in irradiated callus by 8 times, phosphatidylethonolamine by 2 times, and the content of phosphatidic acid decreased by 20% of the sum of PL. For sterols it was found that irradiation caused the most significant changes in the content of β-sitosterol dominant in plants (increase from 1453 ± 170 μg/g dry weight in unirradiated control to 2001 ± 111 μg/g dry weight 1 h after exposure); due to this, the total content of sterols also increased. The analysis of the obtained results suggests that PLs and sterols, primarily those for which regulatory and signaling functions are known, participate in the reaction of plant tissue to exposure to low-intensity He-Ne laser irradiation. This participation is realized as a stress (nonspecific) response to intense radiation.

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ACKNOWLEDGMENTS

The work was performed on the equipment of the Bioanalytical Center of the Siberian Institute of Plant Physiology and Biochemistry Siberian Branch of the Russian Academy of Sciences (Irkutsk).

Funding

The work was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation for Siberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of RAS (project state registration Nо. 0277-2022-0006).

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of the Russian Academy of Sciences, 664033, Irkutsk, Russia

    L. V. Dudareva, E. G. Rudikovskaya, N. V. Semenova, A. V. Rudikovskii & V. N. Shmakov

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  1. L. V. Dudareva
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  2. E. G. Rudikovskaya
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  3. N. V. Semenova
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Correspondence to E. G. Rudikovskaya.

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Dudareva, L.V., Rudikovskaya, E.G., Semenova, N.V. et al. Influence of Low-Intensive He-Ne Laser Radiation on the Composition and Content of Phospholipids and Sterols in the Callus Tissues of Wheat Тriticum aestivum L.. Biochem. Moscow Suppl. Ser. A 18, 100–109 (2024). https://doi.org/10.1134/S1990747824700120

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