Abstract
The study of the permeability to water and urea of plasma membranes of endothelial cells of the normal cornea and cornea with a pronounced form of keratoconus was carried out. Human corneal endothelial cells were obtained from surgical material. Determination of osmotic water permeability (Pf) of normal and keratoconus endothelial cells did not reveal significant differences in the value of this parameter in both groups, where cells had a similar osmotic water permeability (control Pf = 0.53 ± 0.045 cm/s; keratoconus Pf = 0.63 ± 0.041 cm/s) (n = 25; p ≥ 0.05). The urea permeability coefficients (Pu) in both groups also had no statistically significant differences (control Pu = 0.049 ± 0.003 cm/s; keratoconus Pu = 0.056 ± 0.003 cm/s) (n = 25; p ≥ 0.05). Analysis of cell volume dynamics based on exponential approximation showed that corneal endothelial cells with keratoconus reduced their volume in a hypertonic medium to a greater extent compared to cells of a healthy cornea. An increase in cell volume as a result of isotonic urea inflow in a hypertonic medium also occurred to significantly higher values compared to normal cells. The conclusion was made about significant changes in the mechanism of regulation of the volume of corneal endothelial cells in keratoconus.
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The work was carried out with the support of budget project no. FWNR-2022-0019.
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The idea of the study and planning of the experiments (E.I. Solenov and I.A. Iskakov), collection of data (G.S. Baturina, L.E. Katkova, I.M. Kuzeina, and E.I. Solenov), data processing and manuscript writing (G.S. Baturina, L.E. Katkova, I.M. Kuzeina, and E.I. Solenov), discussion and editing of the manuscript (G.S. Baturina, L.E. Katkova, E.I. Solenov, I.G. Palchikova, and I.A. Iskakov).
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The study was conducted in accordance with the principles of the Helsinki Declaration of the World Medical Association “Ethical Principles of Conducting Scientific medical research with human participation”, Federal Law of the Russian Federation No. 323 FZ dated November 21, 2011 “On the Basics of Protecting the Health of Citizens in the Russian Federation”, as well as the requirements of Federal Law No. 152-FZ dated July 27, 2006 (ed. July 21, 2014) “On personal data” (with amendments and additions, effective from September 1, 2015). The sampling of the material was carried out by employees of the Novosibirsk branch of the S.N. Fedorov NMRC “MNTK “Eye microsurgery” Ministry of Health of Russia. Permission was received from the Bioethical Committee of the Novosibirsk branch of the S.N. Fedorov NMRC “MNTK “Eye microsurgery” to conduct the study. All patients gave written informed consent to the study of biological material and the use of the data obtained for scientific purposes.
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Kuseina, I.M., Katkova, L.E., Baturina, G.S. et al. Corneal Endothelial Cell Volume Regulation Disorders in Keratoconus. Biochem. Moscow Suppl. Ser. A 18, 44–50 (2024). https://doi.org/10.1134/S1990747824700065
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DOI: https://doi.org/10.1134/S1990747824700065