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Features of the Structure and Electrophysiological Properties of a Novel Porin from the Marine Bacterium Marinomonas primoryensis

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Using the method of protein reconstitution into planar bilayer lipid membranes, the electrophysiological properties of a novel porin channel from the marine bacterium Marinomonas primoryensis (MpOmp) were characterized. The main characteristics were determined: the conductivity value of the single MpOmp channel, its selectivity, and the values of the critical closing potential in various media (neutral, weakly acidic, alkaline). Using an in silico approach, the geometric characteristics of the MpOmp pore and the distribution of charges at the mouth and inside the porin channel were predicted.

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Funding

The work was supported by the Russian Foundation for Basic Research (project no. 19-04-00318).

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Authors and Affiliations

  1. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    D. K. Chistyulin, E. A. Zelepuga, V. A. Khomenko, O. Yu. Portnyagina & O. D. Novikova

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  1. D. K. Chistyulin
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  2. E. A. Zelepuga
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  3. V. A. Khomenko
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  4. O. Yu. Portnyagina
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  5. O. D. Novikova
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Correspondence to D. K. Chistyulin.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Dunina-Barkovskaya

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Chistyulin, D.K., Zelepuga, E.A., Khomenko, V.A. et al. Features of the Structure and Electrophysiological Properties of a Novel Porin from the Marine Bacterium Marinomonas primoryensis. Biochem. Moscow Suppl. Ser. A 16, 175–179 (2022). https://doi.org/10.1134/S1990747822030047

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  • DOI: https://doi.org/10.1134/S1990747822030047

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