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Responsiveness of HEK 293 Cells to Norepinephrine Analyzed by the On-line Monitoring of Intracellular Ca2+ and cAMP

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Norepinephrine plays an important role in the regulation of a variety of physiological processes. Cells of various types recognize this hormone by employing adrenergic receptors that can be coupled to the phospholipase and adenylate cyclase signaling pathways. Here we investigated the adrenergic system of HEK 293 cells using genetically encoded sensors GEM-GECO1 and Pink Flamindo for on-line monitoring of intracellular concentrations of Ca2+ and cAMP, respectively. We found that in single HEK 293 cells, norepinephrine stimulated both Ca2+ signaling and cAMP production. An interesting distinction between the cAMP and Ca2+ signals was that cAMP responses increased in magnitude with norepinephrine dose, while Ca2+ responses were similarly shaped at different agonist concentrations above the threshold value. The physiological significance of such dose dependencies is discussed.

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ACKNOWLEDGMENTS

The work was supported by the Russian Science Foundation (project no. 19-75-10068).

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, FRC PSCBR RAS, 142290, Pushchino, Moscow oblast, Russia

    E. N. Kochkina, O. A. Rogachevskaja & P. D. Kotova

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  1. E. N. Kochkina
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  2. O. A. Rogachevskaja
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  3. P. D. Kotova
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Correspondence to E. N. Kochkina.

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The authors declare that they have no conflict of interest.

This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Dunina-Barkovskaya

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Kochkina, E.N., Rogachevskaja, O.A. & Kotova, P.D. Responsiveness of HEK 293 Cells to Norepinephrine Analyzed by the On-line Monitoring of Intracellular Ca2+ and cAMP. Biochem. Moscow Suppl. Ser. A 15, 406–409 (2021). https://doi.org/10.1134/S1990747821060052

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  • DOI: https://doi.org/10.1134/S1990747821060052

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