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Identification of sterol-containing domains in vacuolar membranes by confocal microscopy

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Sterol-containing domains in the tonoplast of beet root cells were identified by confocal microscopy with a membrane probe filipin. The presence of sterol-containing domains in the tonoplast was confirmed using methyl-β-cyclodextrin. Sterol-containing domains with separate small intensely fluorescing sterol-enriched sites were found in the vacuolar membrane; these sites can be attributed to raft structures. It was shown that sterol content in the isolated rafts can be assessed by confocal microscopy. β-Sitosterol, stigmasterol, α-tocopherol, and cholesterol were identified among the sterols of the isolated rafts.

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, 664033, Russia

    V. N. Nurminsky, I. S. Nesterkina, E. V. Spiridonova & N. V. Ozolina

  2. Institute of Laser Physics (Irkutsk Department), Siberian Branch, Russian Academy of Sciences, Irkutsk, 664033, Russia

    A. L. Rakevich

Authors
  1. V. N. Nurminsky
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  2. I. S. Nesterkina
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  3. E. V. Spiridonova
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  4. A. L. Rakevich
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  5. N. V. Ozolina
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Correspondence to V. N. Nurminsky.

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Original Russian Text © V.N. Nurminsky, I.S. Nesterkina, E.V. Spiridonova, A.L. Rakevich, N.V. Ozolina, 2017, published in Biologicheskie Membrany, 2017, Vol. 34, No. 4, pp. 307–312.

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Nurminsky, V.N., Nesterkina, I.S., Spiridonova, E.V. et al. Identification of sterol-containing domains in vacuolar membranes by confocal microscopy. Biochem. Moscow Suppl. Ser. A 11, 296–300 (2017). https://doi.org/10.1134/S1990747817040080

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  • DOI: https://doi.org/10.1134/S1990747817040080

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