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Traumatic shock causes elevation of the serotonin 5-HT2B receptor mRNA level in rat aorta

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Previously we have shown that at traumatic shock in rats the force of contraction of isolated aorta in response to angiotensin II, vasopressin, endothelin 1, or norepinephrine is decreased. On the contrary, vasoconstriction caused by serotonin is increased. A possible reason of the alterations of neuroendocrine regulation of vascular tone in shock may be a change in the expression of the receptors of these agonists in blood vessels. In the present study, using real-time PCR, we demonstrated that a day after injury the contents of mRNA encoding receptors V1A for vasopressin, ETA for endothelin 1, and AT1 for angiotensin II are not changed in aorta. There was a slight increase of the serotonin 5-HT2A receptor mRNA (36 ± 16%; p = 0.41). The level of the 5-HT2B receptor mRNA in aorta, initially low (2% of the content of the mRNA of receptors 5-HT2A), after the injury increased 15.8 ± 0.3 times (p < 0.01). However, at traumatic shock there was no contraction of aorta in response to 5-HT2B receptor agonist BW723C86, while vasodilation of the isolated aorta preconstricted with norepinephrine in response to BW723C86 was similar to that of the vessel isolated from control rats. The data obtained suggest that the observed 5-HT2B receptor overexpression is not related to the increased serotonin-induced vasoconstriction and might cause other vascular pathological changes at traumatic shock.

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Authors and Affiliations

  1. Institute of General Pathology and Pathophysiology, ul. Baltiyskaya 8, Moscow, 125315, Russia

    L. M. Kozhevnikova & I. L. Zharkikh

  2. Koltzov Institute of Developmental Biology, ul. Vavilova 26, Moscow, 119334, Russia

    P. P. Avdonin & P. V. Avdonin

Authors
  1. L. M. Kozhevnikova
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  2. P. P. Avdonin
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  3. I. L. Zharkikh
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  4. P. V. Avdonin
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Correspondence to P. V. Avdonin.

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Original Russian Text © L.M. Kozhevnikova, P.P. Avdonin, I.L. Zharkikh, P.V. Avdonin, 2016, published in Biologicheskie Membrany, 2016, Vol. 33, No. 6, pp. 445–449.

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Kozhevnikova, L.M., Avdonin, P.P., Zharkikh, I.L. et al. Traumatic shock causes elevation of the serotonin 5-HT2B receptor mRNA level in rat aorta. Biochem. Moscow Suppl. Ser. A 11, 82–86 (2017). https://doi.org/10.1134/S1990747816040140

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  • DOI: https://doi.org/10.1134/S1990747816040140

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