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Mathematical simulation of H+-sucrose symporter of plasma membrane in higher plants

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

H+-sucrose symporter plays an important role in sucrose loading from apoplast to sieve elements, which is one of the initial steps of carbohydrate transport through the plant. Mechanisms of such transport require experimental and theoretical investigation, including development of the optimal H+-sucrose symporter mathematical model. We carried out comparative analysis of the effectiveness of 1-, 2-, 4- and 6-state symporter models for a quantitative and qualitative sucrose transport description. The proposed models of H+-sucrose symporter were described as components of the general model of sucrose transport in plasma membrane vesicle for adequate comparison with experimental data. The theoretical analysis showed that all four H+-sucrose symporter models (with different level of details) describe experimentally observed dynamics of sucrose efflux, membrane potential change in inverted vesicles, and absolute sucrose efflux properly describe experimentally observed dynamics of sucrose efflux, membrane potential change in inverted vesicles, and absolute sucrose efflux properly. Our results showed that mathematical model of H+-sucrose symporter with 1 state is the optimal model, at least for the investigation of sucrose transport in vesicles.

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Authors and Affiliations

  1. Department of Biophysics, Lobachevsky State University of Nizhny Novgorod, Gagarin pr., 32, Nizhny Novgorod, 603950, Russia

    V. S. Sukhov, V. A. Kalinin, L. M. Surova, O. N. Sherstneva & V. A. Vodeneev

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  1. V. S. Sukhov
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  2. V. A. Kalinin
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  3. L. M. Surova
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  4. O. N. Sherstneva
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  5. V. A. Vodeneev
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Correspondence to V. S. Sukhov.

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Original Russian Text © V.S. Sukhov, V.A. Kalinin, L.M. Surova, O.N. Sherstneva, V.A. Vodeneev, 2013, published in Biologicheskie Membrany, 2013, Vol. 30, No. 2, pp. 128–135.

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Sukhov, V.S., Kalinin, V.A., Surova, L.M. et al. Mathematical simulation of H+-sucrose symporter of plasma membrane in higher plants. Biochem. Moscow Suppl. Ser. A 7, 163–169 (2013). https://doi.org/10.1134/S1990747813010078

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  • DOI: https://doi.org/10.1134/S1990747813010078

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