Abstract
Transduction of many cellular signals is mediated by special plasma membrane microdomains that are called lipid rafts. Lipid rafts are dynamic and transient structures; however, they can be stabilized by lipid raft proteins, including the family of flotillins which is represented by flotillin-1 and flotillin-2. Flotillins are expressed in different tissues and may regulate many signalling pathways. However, their role in carcinogenesis remains unclear. The aim of this work was to investigate variations of flotillin-2 expression on mRNA and protein level in lung adenocarcinoma specimens. We also studied the influence of flotillin-2 expression on the characteristics of A549 lung cancer cell line. The level of flotillin-2 mRNA was reduced in the vast majority of investigated adenocarcinoma specimens in comparison to corresponding normal tissues. However, the amount of protein varied widely and was preferentially increased (40%) than decreased (15%). Flotillin-2 overexpression in A549 cell line did not change proliferation but stimulated migration of cultivated cells. Conversely, knockdown of flotillin-2 using small hairpin RNA (shRNA) downregulated proliferation as well as migration of tumor cells. These results indicate that the expression of flotillin-2 changes in human lung adenocarcinoma and that this protein may influence the key characteristics of tumor cells.
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Original Russian Text © A.N. Shneyderman, K.K. Laktionov, B.E. Polotzky, I.B. Zborovskaya, 2012, published in Biologicheskie Membrany, 2012, Vol. 29, No. 4, pp. 293–304.
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Shneyderman, A.N., Laktionov, K.K., Polotzky, B.E. et al. Variations of expression of lipid raft protein flotillin-2 in human lung adenocarcinomas and its influence on the characteristics of the lung cancer cell line A549. Biochem. Moscow Suppl. Ser. A 6, 300–309 (2012). https://doi.org/10.1134/S199074781203018X
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DOI: https://doi.org/10.1134/S199074781203018X