The priming effect of halogenated phospholipids on the functional responses of human neutrophils

  • I. V. GorudkoEmail author
  • T. V. Vakhrusheva
  • A. V. Mukhortova
  • S. N. Cherenkevich
  • A. V. Timoshenko
  • V. I. Sergienko
  • O. M. Panasenko


Halogenated lipids formed in the reactions with myeloperoxidase (MPO)-derived species may contribute to the regulation of the functional activity of cells. In the present study we have investigated the effects of chloro- and bromohydrins formed in the HOCl and HOBr reactions, respectively, with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) on three different functional responses of human neutrophils: H2O2 generation, degranulation (MPO exocytosis), and cell aggregation. It was shown that POPC chloro- and bromohydrins (POPC-Cl and POPC-Br) induced the priming of neutrophils, resulting in significant upregulation of cell responses to neutrophil stimulators such as N-formyl-Met-Leu-Phe and lectin from Solanum tuberosum. The stimulating effects of POPC-Cl and POPC-Br were observed at low micromolar concentrations (liposomal concentration of POPC, 0.5–5 μM; the content of POPC-Cl or POPC-Br, 38 ± 3% of total lipids) after a short exposure (about 5 min) of the neutrophils to POPC-Cl or POPC-Br. These results suggest that halogenated lipids formed in vivo via MPO-dependent reactions may be considered as a new class of biologically active substances that are potentially able to contribute to the priming of myeloid cells in the sites of inflammation and serve as inflammatory response modulators.

Key words

halogenated phospholipids myeloperoxidase neutrophils priming H2O2 generation degranulation aggregation 



reactive oxygen species


low-density lipoproteins


HOCl-modified LDL








(matrix assisted laser desorption/ionisation time-of-flight), mass spectrometry




liposomes formed of POPC


POPC chlorohydrins


POPC bromohydrins


lectin of potato tubers (Solanum tuberosum)


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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • I. V. Gorudko
    • 1
    Email author
  • T. V. Vakhrusheva
    • 2
  • A. V. Mukhortova
    • 1
  • S. N. Cherenkevich
    • 1
  • A. V. Timoshenko
    • 3
  • V. I. Sergienko
    • 2
  • O. M. Panasenko
    • 2
  1. 1.Department of BiophysicsBelarussian State UniversityMinskBelarus
  2. 2.Research Institute of Physico-Chemical MedicineMoscowRussia
  3. 3.Department of BiologyUniversity of Western OntarioLondonCanada

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