Abstract
The method of atomic force microscopy has been used to investigate the morphology of mica-supported bilayer lipid membranes and stability of their complexes with a cationic polymer, poly-(N-ethyl-4-vinylpyridinium bromide). Lipid bilayers with a minimum of defects were obtained by the fusion of monolamellar neutral or mixed anionic bilayer vesicles (liposomes) on the mica surface, followed by excessive solvent removal by means of rapid rotation of a plate in horizontal plane (spin-coating). It has been shown that the cationic polymer does not interact with the bilayers, where the outer leaflet (i.e., the monolayer exposed to the surrounding aqueous solution) is made of an electroneutral phosphatidylcholine (PC). At the same time, the polymer irreversibly binds to the bilayer containing an anionic lipid.
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Original Russian Text © A.V. Sybachin, L.A. Tsarkova, A.A. Yaroslavov, 2010, published in Biologicheskie Membrany, 2010, Vol. 27, No. 2, pp. 218–224.
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Sybachin, A.V., Tsarkova, L.A. & Yaroslavov, A.A. Atomic force microscopy of supported lipid membranes and their complexes with polycations. Biochem. Moscow Suppl. Ser. A 4, 240–246 (2010). https://doi.org/10.1134/S1990747810020170
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DOI: https://doi.org/10.1134/S1990747810020170