Abstract
The possible role of STIM1 protein in the regulation of activity of receptor- and store-operated Ca2+ channels in non-excitable cells has been studied. Receptor- and store-operated Ca2+ influxes have been measured using the fluorescent method of detection of cytosolic Ca2+ concentration and the electrophysiological methods of whole-cell and single-channel current recordings in the control HEK293 cells and in HEK293 cells with suppressed expression of STIM1. The experiments have shown that STIM1 suppression results in a reduction of the amplitudes of both receptor- and store-operated inward calcium currents. The decrease of total Ca2+ influx of in response to an agonist or to passive depletion of calcium stores upon STIM1 suppression was due to the decrease or total absence of the activity of high-conductance channels Imax and non-selective channels Ins in HEK293 cells. A decrease in the STIM1 amount also altered the activity regulation of low-conductance Imin channels that changed from exclusively agonist-operated into store-dependent channels in HEK293 cells.
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Original Russian Text © O.A. Zimina, V.A. Vigont, I.A. Pozdnjakov, L.N. Glushankova, S.V. L’vovskaja, A.Yu. Skopin, G.N. Mozhayeva, E.V. Kaznacheeva, 2010, published in Biologicheskie Membrany, 2010, Vol. 27, No. 3, pp. 237–243.
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Zimina, O.A., Vigont, V.A., Pozdnjakov, I.A. et al. The role of STIM1 in the receptor- and store-operated calcium influx in HEK293 cells. Biochem. Moscow Suppl. Ser. A 4, 200–205 (2010). https://doi.org/10.1134/S199074781002011X
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DOI: https://doi.org/10.1134/S199074781002011X